Chapter title |
Flow cytometry in environmental microbiology: a rapid approach for the isolation of single cells for advanced molecular biology analysis.
|
---|---|
Chapter number | 1 |
Book title |
Microbial Systems Biology
|
Published in |
Methods in molecular biology, January 2012
|
DOI | 10.1007/978-1-61779-827-6_1 |
Pubmed ID | |
Book ISBNs |
978-1-61779-826-9, 978-1-61779-827-6
|
Authors |
Ferrari BC, Winsley TJ, Bergquist PL, Van Dorst J, Ferrari, Belinda C, Winsley, Tristrom J, Bergquist, Peter L, Van Dorst, Josie, Ali Navid, Belinda C. Ferrari, Tristrom J. Winsley, Peter L. Bergquist, Josie Van Dorst, Ferrari, Belinda C., Winsley, Tristrom J., Bergquist, Peter L. |
Editors |
Ali Navid |
Abstract |
The isolation and subsequent characterization of microbial cells from within environmental samples is a difficult process. Flow cytometry and cell sorting, when combined with the application of fluorescent probes, have the capability for the detection and separation of diverse microbial populations from within complex mixtures. The isolation of single cells allows for downstream investigations towards system-level characterization of unknown Bacterial Phyla to occur. We describe here the combination of fluorescent in situ hybridization and cell sorting for the detection and isolation of Candidate Division TM7 bacteria from an enriched soil sample. The result is the isolation of rare cells suitable for advanced molecular analysis including whole genome amplification and high-throughput pyrosequencing. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Iran, Islamic Republic of | 1 | 3% |
Japan | 1 | 3% |
Mexico | 1 | 3% |
Unknown | 33 | 92% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 10 | 28% |
Researcher | 6 | 17% |
Student > Master | 5 | 14% |
Student > Bachelor | 4 | 11% |
Professor | 2 | 6% |
Other | 6 | 17% |
Unknown | 3 | 8% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 14 | 39% |
Environmental Science | 6 | 17% |
Biochemistry, Genetics and Molecular Biology | 5 | 14% |
Unspecified | 2 | 6% |
Earth and Planetary Sciences | 1 | 3% |
Other | 2 | 6% |
Unknown | 6 | 17% |