| Chapter title |
Molecular Targeted Viral Nanoparticles as Tools for Imaging Cancer
|
|---|---|
| Chapter number | 16 |
| Book title |
Virus Hybrids as Nanomaterials
|
| Published in |
Methods in molecular biology, January 2014
|
| DOI | 10.1007/978-1-62703-751-8_16 |
| Pubmed ID | |
| Book ISBNs |
978-1-62703-750-1, 978-1-62703-751-8
|
| Authors |
Choi-Fong Cho, Sourabh Shukla, Emily J. Simpson, Nicole F. Steinmetz, Leonard G. Luyt, John D. Lewis, Cho CF, Shukla S, Simpson EJ, Steinmetz NF, Luyt LG, Lewis JD, Cho, Choi-Fong, Shukla, Sourabh, Simpson, Emily J, Steinmetz, Nicole F, Luyt, Leonard G, Lewis, John D, Simpson, Emily J., Steinmetz, Nicole F., Luyt, Leonard G., Lewis, John D. |
| Editors |
Baochuan Lin, Banahalli Ratna |
| Abstract |
Viral nanoparticles (VNPs) are a novel class of bionanomaterials that harness the natural biocompatibility of viruses for the development of therapeutics, vaccines, and imaging tools. The plant virus, cowpea mosaic virus (CPMV), has been successfully engineered to create novel cancer-targeted imaging agents by incorporating fluorescent dyes, polyethylene glycol (PEG) polymers, and targeting moieties. Using straightforward conjugation strategies, VNPs with high selectivity for cancer-specific molecular targets can be synthesized for in vivo imaging of tumors. Here we describe the synthesis and purification of CPMV-based VNPs, the functionalization of these VNPs using click chemistry, and their use for imaging xenograft tumors in animal models. VNPs decorated with fluorescent dyes, PEG, and targeting ligands can be synthesized in one day, and imaging studies can be performed over hours, days, or weeks, depending on the application. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 21 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 5 | 24% |
| Researcher | 4 | 19% |
| Student > Bachelor | 2 | 10% |
| Student > Postgraduate | 2 | 10% |
| Professor > Associate Professor | 2 | 10% |
| Other | 3 | 14% |
| Unknown | 3 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 4 | 19% |
| Agricultural and Biological Sciences | 3 | 14% |
| Chemistry | 3 | 14% |
| Immunology and Microbiology | 2 | 10% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 5% |
| Other | 3 | 14% |
| Unknown | 5 | 24% |