Chapter title |
Profiling Abscisic Acid-Induced Changes in Fatty Acid Composition in Mosses
|
---|---|
Chapter number | 18 |
Book title |
Plant Stress Tolerance
|
Published in |
Methods in molecular biology, July 2017
|
DOI | 10.1007/978-1-4939-7136-7_18 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7134-3, 978-1-4939-7136-7
|
Authors |
Suhas Shinde, Shivakumar Devaiah, Aruna Kilaru, Shinde, Suhas, Devaiah, Shivakumar, Kilaru, Aruna |
Abstract |
In plants, change in lipid composition is a common response to various abiotic stresses. Lipid constituents of bryophytes are of particular interest as they differ from that of flowering plants. Unlike higher plants, mosses have high content of very long-chain polyunsaturated fatty acids. Such lipids are considered to be important for survival of nonvascular plants. Here, using abscisic acid (ABA )-induced changes in lipid composition in Physcomitrella patens as an example, a protocol for total lipid extraction and quantification by gas chromatography (GC) coupled with flame ionization detector (FID) is described. |
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Members of the public | 1 | 100% |
Mendeley readers
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Unknown | 11 | 100% |
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Student > Ph. D. Student | 4 | 36% |
Other | 1 | 9% |
Student > Master | 1 | 9% |
Researcher | 1 | 9% |
Professor > Associate Professor | 1 | 9% |
Other | 0 | 0% |
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Chemistry | 2 | 18% |
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Chemical Engineering | 1 | 9% |
Unknown | 4 | 36% |