Chapter title |
Determining Glutathione Levels in Plants
|
---|---|
Chapter number | 16 |
Book title |
Plant Stress Tolerance
|
Published in |
Methods in molecular biology, July 2017
|
DOI | 10.1007/978-1-4939-7136-7_16 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7134-3, 978-1-4939-7136-7
|
Authors |
Smita Sahoo, Jay Prakash Awasthi, Ramanjulu Sunkar, Sanjib Kumar Panda, Sahoo, Smita, Awasthi, Jay Prakash, Sunkar, Ramanjulu, Panda, Sanjib Kumar |
Abstract |
Upon exposure to abiotic stresses, plants tend to accumulate excessive amounts of reactive oxygen species (ROS) that inturn react with cellular lipids, proteins, and DNA. Therefore, decreasing ROS accumulation is indispensible to survive under stress, which is accomplished by inducing enzymatic and nonenzymatic antioxidant defense pathways. Glutathione, particularly reduced glutathione (GSH), represents a principal anitioxidant that could decrease ROS through scavenging them directly or indirectly through ascorbate-glutathione cycle or GSH peroxidases. Glutathione content can be determined using HPLC or spectrophotometric assays. In this chapter, we provided detailed assays to determine total, reduced, and oxidized gluathione using spectrophotometric method. |
X Demographics
Geographical breakdown
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 52 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Master | 11 | 21% |
Researcher | 10 | 19% |
Student > Ph. D. Student | 9 | 17% |
Other | 4 | 8% |
Unspecified | 2 | 4% |
Other | 5 | 10% |
Unknown | 11 | 21% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 16 | 31% |
Biochemistry, Genetics and Molecular Biology | 6 | 12% |
Environmental Science | 4 | 8% |
Engineering | 3 | 6% |
Unspecified | 2 | 4% |
Other | 4 | 8% |
Unknown | 17 | 33% |