Chapter title |
Imaging of Dynamic Ion Signaling During Root Gravitropism
|
---|---|
Chapter number | 5 |
Book title |
Plant Gravitropism
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2697-8_5 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2696-1, 978-1-4939-2697-8
|
Authors |
Gabriele B. Monshausen |
Abstract |
Gravitropic signaling is a complex process that requires the coordinated action of multiple cell types and tissues. Ca(2+) and pH signaling are key components of gravitropic signaling cascades and can serve as useful markers to dissect the molecular machinery mediating plant gravitropism. To monitor dynamic ion signaling, imaging approaches combining fluorescent ion sensors and confocal fluorescence microscopy are employed, which allow the visualization of pH and Ca(2+) changes at the level of entire tissues, while also providing high spatiotemporal resolution. Here, I describe procedures to prepare Arabidopsis seedlings for live cell imaging and to convert a microscope for vertical stage fluorescence microscopy. With this imaging system, ion signaling can be monitored during all phases of the root gravitropic response. |
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Geographical breakdown
Country | Count | As % |
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Unknown | 6 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 1 | 17% |
Professor > Associate Professor | 1 | 17% |
Researcher | 1 | 17% |
Unknown | 3 | 50% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 3 | 50% |
Unknown | 3 | 50% |