| Chapter title |
Proteomic Approaches and Their Application to Plant Gravitropism
|
|---|---|
| Chapter number | 10 |
| Book title |
Plant Gravitropism
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-2697-8_10 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2696-1, 978-1-4939-2697-8
|
| Authors |
Proma Basu, Darron R. Luesse, Sarah E. Wyatt, Basu, Proma, Luesse, Darron R., Wyatt, Sarah E. |
| Abstract |
Proteomics is a powerful technique that allows researchers a window into how an organism responds to a mutation, a specific environment, or at a distinct point during development by quantifying relative protein abundance and posttranslational modifications. Here, we describe methods for the proteomic analysis of Arabidopsis thaliana tissue. Extraction protocols are provided for isolation of soluble, plasma membrane, and tonoplast proteins. In addition, basic analysis and quality metrics for MS/MS data are discussed. The protocols outlined have the potential to unlock new avenues of research that are not possible through basic genetics or transcriptomic approaches. By combining proteomic information with known gene regulatory patterns, researchers can gain a complete picture of how molecular pathways, such as those required for gravitropism, are initiated, regulated, and terminated. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 5 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Unspecified | 1 | 20% |
| Librarian | 1 | 20% |
| Student > Ph. D. Student | 1 | 20% |
| Unknown | 2 | 40% |
| Readers by discipline | Count | As % |
|---|---|---|
| Unspecified | 1 | 20% |
| Biochemistry, Genetics and Molecular Biology | 1 | 20% |
| Unknown | 3 | 60% |