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Mendeley readers
Chapter title |
Detecting Protein ADP-Ribosylation Using a Clickable Aminooxy Probe
|
---|---|
Chapter number | 6 |
Book title |
Poly(ADP-Ribose) Polymerase
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Published in |
Methods in molecular biology, July 2017
|
DOI | 10.1007/978-1-4939-6993-7_6 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6992-0, 978-1-4939-6993-7
|
Authors |
Rory K. Morgan, Michael S. Cohen |
Abstract |
ADP-ribosylation, a posttranslational modification catalyzed by a family of enzymes known as poly(ADP-ribose) polymerases (PARPs, 17 in humans), regulates diverse cellular processes. To aid in understanding the functions of ADP-ribosylation in cells, we developed a clickable aminooxy probe, AO-alkyne, which detects ADP-ribosylation of acidic amino acids. AO-alkyne can be used to detect auto-ADP-ribosylation of PARP10 in cells following Cu-catalyzed click conjugation to an azide reporter. This method can be extended to other PARP family members that catalyze ADP-ribosylation on acidic amino acids, providing a convenient and direct readout of PARP activity in cells. |
Mendeley readers
The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 8 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 2 | 25% |
Professor > Associate Professor | 2 | 25% |
Student > Master | 2 | 25% |
Student > Doctoral Student | 1 | 13% |
Unspecified | 1 | 13% |
Other | 0 | 0% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 2 | 25% |
Veterinary Science and Veterinary Medicine | 1 | 13% |
Unspecified | 1 | 13% |
Agricultural and Biological Sciences | 1 | 13% |
Unknown | 3 | 38% |