| Chapter title |
Alternative sources of adult stem cells: human amniotic membrane.
|
|---|---|
| Chapter number | 71 |
| Book title |
Bioreactor Systems for Tissue Engineering II
|
| Published in |
Advances in biochemical engineering biotechnology, March 2010
|
| DOI | 10.1007/10_2010_71 |
| Pubmed ID | |
| Book ISBNs |
978-3-64-216050-9, 978-3-64-216051-6
|
| Authors |
Wolbank S, van Griensven M, Grillari-Voglauer R, Peterbauer-Scherb A, Susanne Wolbank, Martijn van Griensven, Regina Grillari-Voglauer, Anja Peterbauer-Scherb, Wolbank, Susanne, Griensven, Martijn, Grillari-Voglauer, Regina, Peterbauer-Scherb, Anja |
| Abstract |
Human amniotic membrane is a highly promising cell source for tissue engineering. The cells thereof, human amniotic epithelial cells (hAEC) and human amniotic mesenchymal stromal cells (hAMSC), may be immunoprivileged, they represent an early developmental status, and their application is ethically uncontroversial. Cell banking strategies may use freshly isolated cells or involve in vitro expansion to increase cell numbers. Therefore, we have thoroughly characterized the effect of in vitro cultivation on both phenotype and differentiation potential of hAEC. Moreover, we present different strategies to improve expansion including replacement of animal-derived supplements by human platelet products or the introduction of the catalytic subunit of human telomerase to extend the in vitro lifespan of amniotic cells. Characterization of the resulting cultures includes phenotype, growth characteristics, and differentiation potential, as well as immunogenic and immunomodulatory properties. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 39 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 10 | 26% |
| Student > Ph. D. Student | 9 | 23% |
| Student > Master | 3 | 8% |
| Other | 2 | 5% |
| Professor > Associate Professor | 2 | 5% |
| Other | 5 | 13% |
| Unknown | 8 | 21% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 9 | 23% |
| Biochemistry, Genetics and Molecular Biology | 7 | 18% |
| Agricultural and Biological Sciences | 5 | 13% |
| Chemical Engineering | 2 | 5% |
| Nursing and Health Professions | 1 | 3% |
| Other | 3 | 8% |
| Unknown | 12 | 31% |