Chapter title |
Identification of Small Novel Coding Sequences, a Proteogenomics Endeavor
|
---|---|
Chapter number | 4 |
Book title |
Proteogenomics
|
Published in |
Advances in experimental medicine and biology, January 2016
|
DOI | 10.1007/978-3-319-42316-6_4 |
Pubmed ID | |
Book ISBNs |
978-3-31-942314-2, 978-3-31-942316-6
|
Authors |
Volodimir Olexiouk, Gerben Menschaert, Olexiouk, Volodimir, Menschaert, Gerben |
Abstract |
The identification of small proteins and peptides has consistently proven to be challenging. However, technological advances as well as multi-omics endeavors facilitate the identification of novel small coding sequences, leading to new insights. Specifically, the application of next generation sequencing technologies (NGS), providing accurate and sample specific transcriptome / translatome information, into the proteomics field led to more comprehensive results and new discoveries. This book chapter focuses on the inclusion of RNA-Seq and RIBO-Seq also known as ribosome profiling, an RNA-Seq based technique sequencing the +/- 30 bp long fragments captured by translating ribosomes. We emphasize the identification of micropeptides and neo-antigens, two distinct classes of small translation products, triggering our current understanding of biology. RNA-Seq is capable of capturing sample specific genomic variations, enabling focused neo-antigen identification. RIBO-Seq can identify translation events in small open reading frames which are considered to be non-coding, leading to the discovery of micropeptides. The identification of small translation products requires the integration of multi-omics data, stressing the importance of proteogenomics in this novel research area. |
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Members of the public | 1 | 100% |
Mendeley readers
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Unknown | 37 | 100% |
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Student > Master | 7 | 19% |
Student > Ph. D. Student | 6 | 16% |
Other | 4 | 11% |
Researcher | 3 | 8% |
Student > Bachelor | 3 | 8% |
Other | 7 | 19% |
Unknown | 7 | 19% |
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Business, Management and Accounting | 1 | 3% |
Other | 3 | 8% |
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