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Hematopoietic Stem Cell Protocols

Overview of attention for book
Cover of 'Hematopoietic Stem Cell Protocols'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 The Hematopoietic Stem Cell Landscape
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    Chapter 2 Investigating the Interaction Between Hematopoietic Stem Cells and Their Niche During Embryonic Development: Optimizing the Isolation of Fetal and Newborn Stem Cells From Liver, Spleen, and Bone Marrow
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    Chapter 3 Hematopoietic Stem Cell Protocols
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    Chapter 4 Hematopoietic Stem and Progenitor Cell Mobilization in Mice
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    Chapter 5 Cell cycle measurement of mouse hematopoietic stem/progenitor cells.
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    Chapter 6 Flow Cytometric Analysis of Signaling and Apoptosis in Hematopoietic Stem Cells
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    Chapter 7 Gene Expression Profiling of Hematopoietic Stem Cells (HSCs)
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    Chapter 8 Measuring MicroRNA Expression in Mouse Hematopoietic Stem Cells.
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    Chapter 9 DNA methylation profiling of hematopoietic stem cells.
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    Chapter 10 Metabolic Characterization of Hematopoietic Stem Cells
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    Chapter 11 Nanoproteomic Assays on Hematopoietic Stem Cells
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    Chapter 12 Hematopoietic Differentiation of Pluripotent Stem Cells in Culture
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    Chapter 13 Ex Vivo Assays to Study Self-Renewal, Long-Term Expansion, and Leukemic Transformation of Genetically Modified Human Hematopoietic and Patient-Derived Leukemic Stem Cells
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    Chapter 14 Ex vivo expansion of murine and human hematopoietic stem cells.
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    Chapter 15 Using Microfluidics to Investigate Hematopoietic Stem Cell and Microniche Interactions at the Single Cell Level
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    Chapter 16 Five-Lineage Clonal Analysis of Hematopoietic Stem/Progenitor Cells
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    Chapter 17 Intravital Imaging of Hematopoietic Stem Cells in the Mouse Skull
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    Chapter 18 Immunodeficient Mouse Model for Human Hematopoietic Stem Cell Engraftment and Immune System Development
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    Chapter 19 Homing and Migration Assays of Hematopoietic Stem/Progenitor Cells
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    Chapter 20 Retroviral transduction of murine and human hematopoietic progenitors and stem cells.
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    Chapter 21 Lentiviral Gene Transduction of Mouse and Human Hematopoietic Stem Cells
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    Chapter 22 High-throughput genomic mapping of vector integration sites in gene therapy studies.
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    Chapter 23 Barcoded Vector Libraries and Retroviral or Lentiviral Barcoding of Hematopoietic Stem Cells
Attention for Chapter 9: DNA methylation profiling of hematopoietic stem cells.
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Chapter title
DNA methylation profiling of hematopoietic stem cells.
Chapter number 9
Book title
Hematopoietic Stem Cell Protocols
Published in
Methods in molecular biology, July 2014
DOI 10.1007/978-1-4939-1133-2_9
Pubmed ID
25062626
Book ISBNs
978-1-4939-1132-5, 978-1-4939-1133-2
Authors

Begtrup AH, Amber Hogart Begtrup

Editors

Kevin D. Bunting, Cheng-Kui Qu

Abstract

DNA methylation is a key epigenetic mark that is essential for properly functioning hematopoietic stem cells. Determining where functionally relevant DNA methylation marks exist in the genome is crucial to understanding the role that methylation plays in hematopoiesis. This chapter describes a method to profile DNA methylation by selectively enriching methylated DNA sequences that are bound in vitro by methyl-binding domain (MBD) proteins. The MBD-pulldown approach selects for DNA sequences that have the potential to be "read" by the endogenous machinery involved in epigenetic regulation. Furthermore, this approach is feasible with very small quantities of DNA, and is compatible with the use of any downstream high-throughput sequencing approach. This technique offers a reliable, simple, and powerful tool for exploration of the role of DNA methylation in hematopoietic stem cells.

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The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
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Mendeley readers

The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 7 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 2 29%
Student > Bachelor 2 29%
Other 1 14%
Researcher 1 14%
Student > Postgraduate 1 14%
Other 0 0%
Readers by discipline Count As %
Nursing and Health Professions 2 29%
Medicine and Dentistry 2 29%
Biochemistry, Genetics and Molecular Biology 1 14%
Agricultural and Biological Sciences 1 14%
Pharmacology, Toxicology and Pharmaceutical Science 1 14%
Other 0 0%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 July 2014.
All research outputs
#20,236,620
of 22,763,032 outputs
Outputs from Methods in molecular biology
#9,864
of 13,088 outputs
Outputs of similar age
#192,536
of 228,715 outputs
Outputs of similar age from Methods in molecular biology
#36
of 70 outputs
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So far Altmetric has tracked 13,088 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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