Chapter title |
Long-Wavelength X-Ray Diffraction and Its Applications in Macromolecular Crystallography
|
---|---|
Chapter number | 17 |
Book title |
Protein Crystallography
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-7000-1_17 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6998-2, 978-1-4939-7000-1
|
Authors |
Manfred S. Weiss |
Editors |
Alexander Wlodawer, Zbigniew Dauter, Mariusz Jaskolski |
Abstract |
For many years, diffraction experiments in macromolecular crystallography at X-ray wavelengths longer than that of Cu-K α (1.54 Å) have been largely underappreciated. Effects caused by increased X-ray absorption result in the fact that these experiments are more difficult than the standard diffraction experiments at short wavelengths. However, due to the also increased anomalous scattering of many biologically relevant atoms, important additional structural information can be obtained. This information, in turn, can be used for phase determination, for substructure identification, in molecular replacement approaches, as well as in structure refinement. This chapter reviews the possibilities and the difficulties associated with such experiments, and it provides a short description of two macromolecular crystallography synchrotron beam lines dedicated to long-wavelength X-ray diffraction experiments. |
Mendeley readers
Geographical breakdown
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Unknown | 9 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 3 | 33% |
Student > Bachelor | 2 | 22% |
Student > Ph. D. Student | 1 | 11% |
Student > Doctoral Student | 1 | 11% |
Unknown | 2 | 22% |
Readers by discipline | Count | As % |
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Chemistry | 2 | 22% |
Physics and Astronomy | 1 | 11% |
Unknown | 2 | 22% |