Chapter title |
Radiation Damage in Macromolecular Crystallography
|
---|---|
Chapter number | 20 |
Book title |
Protein Crystallography
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-7000-1_20 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6998-2, 978-1-4939-7000-1
|
Authors |
Elspeth F. Garman, Martin Weik |
Editors |
Alexander Wlodawer, Zbigniew Dauter, Mariusz Jaskolski |
Abstract |
Radiation damage inflicted on macromolecular crystals during X-ray diffraction experiments remains a limiting factor for structure solution, even when samples are cooled to cryotemperatures (~100 K). Efforts to establish mitigation strategies are ongoing and various approaches, summarized below, have been investigated over the last 15 years, resulting in a deeper understanding of the physical and chemical factors affecting damage rates. The recent advent of X-ray free electron lasers permits "diffraction-before-destruction" by providing highly brilliant and short (a few tens of fs) X-ray pulses. New fourth generation synchrotron sources now coming on line with higher X-ray flux densities than those available from third generation synchrotrons will bring the issue of radiation damage once more to the fore for structural biologists. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 43 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 10 | 23% |
Student > Ph. D. Student | 9 | 21% |
Student > Bachelor | 6 | 14% |
Professor | 4 | 9% |
Student > Doctoral Student | 2 | 5% |
Other | 4 | 9% |
Unknown | 8 | 19% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 8 | 19% |
Agricultural and Biological Sciences | 7 | 16% |
Chemistry | 7 | 16% |
Physics and Astronomy | 5 | 12% |
Medicine and Dentistry | 4 | 9% |
Other | 2 | 5% |
Unknown | 10 | 23% |