| Chapter title |
In Vitro-Induced Germinal Center B Cell Culture System
|
|---|---|
| Chapter number | 11 |
| Book title |
Germinal Centers
|
| Published in |
Methods in molecular biology, June 2017
|
| DOI | 10.1007/978-1-4939-7095-7_11 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7094-0, 978-1-4939-7095-7, 978-1-4939-7094-0, 978-1-4939-7095-7
|
| Authors |
Haniuda, Kei, Nojima, Takuya, Kitamura, Daisuke, Kei Haniuda, Takuya Nojima, Daisuke Kitamura |
| Abstract |
In germinal centers (GCs), B cells undergo repeated cycles of proliferation and affinity-based selection, and differentiate into memory B cells or long-lived plasma cells. It has been difficult to elucidate regulatory mechanisms for the dynamic GC B cell maturation and differentiation, partly because experimental manipulation of GC B cells has been limited. Here we describe a culture system in which we can induce massive expansion of naive B cells that exhibit GC B cell-like phenotype and acquire abilities to differentiate into memory B cells or bone marrow plasma cells depending on cytokine conditions. This system will allow us to elucidate the molecular mechanisms of GC B cell differentiation. |
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