| Chapter title |
Cytokine Expression by T Follicular Helper Cells
|
|---|---|
| Chapter number | 8 |
| Book title |
Germinal Centers
|
| Published in |
Methods in molecular biology, June 2017
|
| DOI | 10.1007/978-1-4939-7095-7_8 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7094-0, 978-1-4939-7095-7
|
| Authors |
Jandl, Christoph, Loetsch, Claudia, King, Cecile, Christoph Jandl, Claudia Loetsch, Cecile King |
| Abstract |
T follicular helper (Tfh) cells are a specialized subset of CD4(+) T cells located within temporary structures known as germinal centers (GC) formed within B cell follicles of secondary lymphoid organs. In the GC, Tfh cells facilitate the production of high-affinity antibodies through secretion of effector cytokines, such as IL-21 and IL-4, and through cell-to-cell interactions. The flow cytometric-based method described here allows the detection of intracellular cytokines within the Tfh population of secondary lymphoid organs (e.g., spleen, lymph nodes, and lymphoid nodules such as Peyer's patches), enabling the study of Tfh responses to different stimuli in the context of immunity and autoimmunity. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 15 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 5 | 33% |
| Student > Ph. D. Student | 4 | 27% |
| Student > Bachelor | 2 | 13% |
| Student > Master | 2 | 13% |
| Unknown | 2 | 13% |
| Readers by discipline | Count | As % |
|---|---|---|
| Immunology and Microbiology | 6 | 40% |
| Biochemistry, Genetics and Molecular Biology | 3 | 20% |
| Veterinary Science and Veterinary Medicine | 1 | 7% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 7% |
| Agricultural and Biological Sciences | 1 | 7% |
| Other | 1 | 7% |
| Unknown | 2 | 13% |