Title |
Poly(γ-glutamic acid)-coated lipoplexes loaded with Doxorubicin for enhancing the antitumor activity against liver tumors
|
---|---|
Published in |
Discover Nano, May 2017
|
DOI | 10.1186/s11671-017-2081-1 |
Pubmed ID | |
Authors |
Na Qi, Bo Tang, Guang Liu, Xingsi Liang |
Abstract |
The study was to develop poly-γ-glutamic acid (γ-PGA)-coated Doxorubicin (Dox) lipoplexes that enhance the antitumor activity against liver tumors. γ-PGA-coated lipoplexes were performed by electrostatistically attracting to the surface of cationic charge liposomes with anionic γ-PGA. With the increasing of γ-PGA concentration, the particle size of γ-PGA-coated Dox lipoplexes slightly increased, the zeta potential from positive shifted to negative, and the entrapment efficiency (EE) were no significant change. The release rate of γ-PGA-coated Dox lipoplexes slightly increased at acidic pH, the accelerated Dox release might be attributed to greater drug delivery to tumor cells, resulting in a higher antitumor activity. Especially, γ-PGA-coated Dox lipoplexes exhibited higher cellular uptake, significant in vitro cytotoxicity in HepG2 cells, and improved in vivo antitumor efficacy toward HepG2 hepatoma-xenografted nude models in comparison with Dox liposomes and free Dox solution. In addition, the analysis results via flow cytometry showed that γ-PGA-coated Dox lipoplexes induce S phase cell cycle arrest and significantly increased apoptosis rate of HepG2 cells. In conclusion, the presence of γ-PGA on the surface of Dox lipoplexes enhanced antitumor effects of liver tumors. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 13 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 4 | 31% |
Student > Ph. D. Student | 2 | 15% |
Student > Bachelor | 1 | 8% |
Lecturer | 1 | 8% |
Researcher | 1 | 8% |
Other | 0 | 0% |
Unknown | 4 | 31% |
Readers by discipline | Count | As % |
---|---|---|
Chemistry | 3 | 23% |
Agricultural and Biological Sciences | 3 | 23% |
Biochemistry, Genetics and Molecular Biology | 2 | 15% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 8% |
Unknown | 4 | 31% |