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Mendeley readers
Chapter title |
Long Fragment Polymerase Chain Reaction.
|
---|---|
Chapter number | 3 |
Book title |
PCR
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-7060-5_3 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7059-9, 978-1-4939-7060-5
|
Authors |
Eng Wee Chua, Simran Maggo, Martin A. Kennedy, Chua, Eng Wee, Maggo, Simran, Kennedy, Martin A. |
Editors |
Lucília Domingues |
Abstract |
Polymerase chain reaction (PCR) is an oft-used preparatory technique in amplifying specific DNA regions for downstream analysis. The size of an amplicon was initially limited by errors in nucleotide polymerization and template deterioration during thermal cycling. A variant of PCR, designated long-range PCR, was devised to counter these drawbacks and enable the amplification of large fragments exceeding a few kb. In this chapter we describe a protocol for long-range PCR, which we have adopted to obtain products of 6.6, 7.2, 13, and 20 kb from human genomic DNA samples. |
Mendeley readers
The data shown below were compiled from readership statistics for 32 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 32 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 5 | 16% |
Other | 3 | 9% |
Student > Bachelor | 3 | 9% |
Student > Ph. D. Student | 3 | 9% |
Student > Postgraduate | 3 | 9% |
Other | 4 | 13% |
Unknown | 11 | 34% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 9 | 28% |
Agricultural and Biological Sciences | 7 | 22% |
Pharmacology, Toxicology and Pharmaceutical Science | 2 | 6% |
Computer Science | 1 | 3% |
Immunology and Microbiology | 1 | 3% |
Other | 2 | 6% |
Unknown | 10 | 31% |