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Mendeley readers
Attention Score in Context
| Chapter title |
PCR in Metagenomics
|
|---|---|
| Chapter number | 17 |
| Book title |
PCR
|
| Published in |
Methods in molecular biology, March 2017
|
| DOI | 10.1007/978-1-4939-7060-5_17 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7059-9, 978-1-4939-7060-5
|
| Authors |
Johny, Tina Kollannoor, Bhat, Sarita Ganapathy, Tina Kollannoor Johny, Sarita Ganapathy Bhat |
| Editors |
Lucília Domingues |
| Abstract |
Metagenomics approach involves direct genetic analysis of environmental samples, evading the tedious culturing process. Polymerase chain reaction is one invaluable tool used for such analyses. Here, we describe one protocol for metagenomic DNA isolation that gives inhibitor-free DNA suitable for PCR and other genetic manipulations. Subsequently, the chapter describes the use of PCR as an indicator of quality of DNA and to amplify a marker of phylogeny. Further, the application of PCR for detection of specific genes and screening of metagenomic libraries is outlined. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 22 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 22 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 4 | 18% |
| Researcher | 3 | 14% |
| Student > Doctoral Student | 2 | 9% |
| Student > Bachelor | 1 | 5% |
| Unknown | 12 | 55% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 6 | 27% |
| Agricultural and Biological Sciences | 3 | 14% |
| Environmental Science | 1 | 5% |
| Medicine and Dentistry | 1 | 5% |
| Unknown | 11 | 50% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 May 2017.
All research outputs
#15,821,622
of 23,498,099 outputs
Outputs from Methods in molecular biology
#5,527
of 13,368 outputs
Outputs of similar age
#195,243
of 309,646 outputs
Outputs of similar age from Methods in molecular biology
#108
of 299 outputs
Altmetric has tracked 23,498,099 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,368 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 44th percentile – i.e., 44% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 309,646 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 28th percentile – i.e., 28% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 299 others from the same source and published within six weeks on either side of this one. This one is in the 49th percentile – i.e., 49% of its contemporaries scored the same or lower than it.