Chapter title |
Harnessing the Power of PCR Molecular Fingerprinting Methods and Next Generation Sequencing for Understanding Structure and Function in Microbial C... - PubMed - NCBI
|
---|---|
Chapter number | 16 |
Book title |
PCR
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-7060-5_16 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7059-9, 978-1-4939-7060-5
|
Authors |
Phadke, Sujal, Salvador, Andreia Filipa, Alves, Joana Isabel, Bretschger, Orianna, Alves, Maria Madalena, Pereira, Maria Alcina, Sujal Phadke, Andreia Filipa Salvador, Joana Isabel Alves, Orianna Bretschger, Maria Madalena Alves, Maria Alcina Pereira |
Editors |
Lucília Domingues |
Abstract |
Polymerase chain reaction (PCR) is central to methods in molecular ecology. Here, we describe PCR-dependent approaches useful for investigating microbial diversity and its function in various natural, human-associated, and built environment ecosystems. Protocols routinely used for DNA extraction, purification, cloning, and sequencing are included along with various resources for the statistical analysis following gel electrophoresis-based methods (DGGE) and sequencing. We also provide insights into eukaryotic microbiome analysis, sample preservation techniques, PCR troubleshooting, DNA quantification methods, and commonly used ordination techniques. |
X Demographics
As of 1 July 2024, you may notice a temporary increase in the numbers of X profiles with Unknown location. Click here to learn more.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 3 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 3 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 10 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 3 | 30% |
Professor | 1 | 10% |
Student > Doctoral Student | 1 | 10% |
Student > Ph. D. Student | 1 | 10% |
Researcher | 1 | 10% |
Other | 0 | 0% |
Unknown | 3 | 30% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 4 | 40% |
Environmental Science | 1 | 10% |
Medicine and Dentistry | 1 | 10% |
Engineering | 1 | 10% |
Unknown | 3 | 30% |