Chapter title |
Flow Cytometric Analysis of the Expression Pattern of Peroxisomal Proteins, Abcd1, Abcd2, and Abcd3 in BV-2 Murine Microglial Cells
|
---|---|
Chapter number | 25 |
Book title |
Peroxisomes
|
Published in |
Methods in molecular biology, April 2017
|
DOI | 10.1007/978-1-4939-6937-1_25 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6935-7, 978-1-4939-6937-1
|
Authors |
Meryam Debbabi, Thomas Nury, Imen Helali, El Mostafa Karym, Flore Geillon, Catherine Gondcaille, Doriane Trompier, Amina Najid, Sébastien Terreau, Maryem Bezine, Amira Zarrouk, Anne Vejux, Pierre Andreoletti, Mustapha Cherkaoui-Malki, Stéphane Savary, Gérard Lizard Ph.D., Debbabi, Meryam, Nury, Thomas, Helali, Imen, Karym, El Mostafa, Geillon, Flore, Gondcaille, Catherine, Trompier, Doriane, Najid, Amina, Terreau, Sébastien, Bezine, Maryem, Zarrouk, Amira, Vejux, Anne, Andreoletti, Pierre, Cherkaoui-Malki, Mustapha, Savary, Stéphane, Lizard, Gérard, Gérard Lizard |
Editors |
Michael Schrader |
Abstract |
Microglial cells play important roles in neurodegenerative diseases including peroxisomal leukodystrophies. The BV-2 murine immortalized cells are widely used in the context of neurodegenerative researches. It is therefore important to establish the expression pattern of peroxisomal proteins by flow cytometry in these cells. So, the expression pattern of various peroxisomal transporters (Abcd1, Abcd2, Abcd3) contributing to peroxisomal β-oxidation was evaluated on BV-2 cells by flow cytometry and complementary methods (fluorescence microscopy, and RT-qPCR). By flow cytometry a strong expression of peroxisomal proteins (Abcd1, Abcd2, Abcd3) was observed. These data were in agreement with those obtained by fluorescence microscopy (presence of numerous fluorescent dots in the cytoplasm characteristic of a peroxisomal staining pattern) and RT-qPCR (high levels of Abcd1, Abcd2, and Abcd3 mRNAs). Thus, the peroxisomal proteins (Abcd1, Abcd2, Abcd3) are expressed in BV-2 cells, and can be analyzed by flow cytometry. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
France | 1 | 50% |
United States | 1 | 50% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
France | 1 | 10% |
Unknown | 9 | 90% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Professor > Associate Professor | 2 | 20% |
Professor | 2 | 20% |
Student > Doctoral Student | 1 | 10% |
Student > Bachelor | 1 | 10% |
Researcher | 1 | 10% |
Other | 1 | 10% |
Unknown | 2 | 20% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 3 | 30% |
Neuroscience | 3 | 30% |
Agricultural and Biological Sciences | 2 | 20% |
Unknown | 2 | 20% |