Chapter title |
siRNA-mediated Silencing of Peroxisomal Genes in Mammalian Cells
|
---|---|
Chapter number | 8 |
Book title |
Peroxisomes
|
Published in |
Methods in molecular biology, April 2017
|
DOI | 10.1007/978-1-4939-6937-1_8 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6935-7, 978-1-4939-6937-1
|
Authors |
Schrader, Tina A., Schrader, Michael, Tina A. Schrader, Michael Schrader |
Editors |
Michael Schrader |
Abstract |
RNAi technologies are a valuable tool in the identification and investigation of proteins that are involved in peroxisome biogenesis and function. Small interfering RNA (siRNA) has developed into the most commonly used RNAi tool for the induction of transient, short-term silencing of protein coding genes. Although siRNA can induce gene knockdown in a variety of mammalian cell lines, their utility is limited by efficient uptake of synthetic oligonucleotides into the cells. Here, we describe different transfection methods that have been successfully used by us to silence peroxisomal genes in a variety of cell lines, including primary human skin fibroblasts, which are usually difficult to transfect. |
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