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Mendeley readers
Chapter title |
Transcription Factors Downstream of IL-4 and TGF-β Signals: Analysis by Quantitative PCR, Western Blot, and Flow Cytometry
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Chapter number | 11 |
Book title |
Th9 Cells
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Published in |
Methods in molecular biology, January 2017
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DOI | 10.1007/978-1-4939-6877-0_11 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6876-3, 978-1-4939-6877-0
|
Authors |
Atsushi Sugimoto, Ryoji Kawakami, Norihisa Mikami |
Editors |
Ritobrata Goswami |
Abstract |
IL-9-producing Th9 cell is a novel Th cell subset involved in type II allergic inflammations such as asthma. Th9 cells can be induced from naïve Th cells in the presence of IL-4 and TGF-β. It is also well established that downstream signals of IL-4 and TGF-β, including STAT6, IRF4, Smad, and PU.1, directly mediate IL-9 production in Th9 cells. In this chapter we describe the methods of flow cytometry, qPCR and western blot analysis to determine the expression or activation of these transcription factors downstream of IL-4 and TGF-β. |
Mendeley readers
The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 3 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Unspecified | 1 | 33% |
Student > Ph. D. Student | 1 | 33% |
Unknown | 1 | 33% |
Readers by discipline | Count | As % |
---|---|---|
Unspecified | 1 | 33% |
Immunology and Microbiology | 1 | 33% |
Unknown | 1 | 33% |