Chapter title |
Potential Involvement of SCF-Complex in Zygotic Genome Activation During Early Bovine Embryo Development
|
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Chapter number | 17 |
Book title |
Zygotic Genome Activation
|
Published in |
Methods in molecular biology, April 2017
|
DOI | 10.1007/978-1-4939-6988-3_17 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6986-9, 978-1-4939-6988-3
|
Authors |
Benesova, Veronika, Kinterova, Veronika, Kanka, Jiri, Toralova, Tereza, Veronika Benesova, Veronika Kinterova, Jiri Kanka, Tereza Toralova |
Editors |
Kiho Lee |
Abstract |
Proper timing of degradation of maternal protein reserves is important for early embryonic development. The major modification that triggers proteins to degradation is ubiquitination, mediated by ubiquitin-proteolytic system. We focus here on Skp 1-Cul 1-F-box complex (SCF-complex), E3 ubiquitin-ligase, a part of ubiquitin-proteolytic system, which transfer ubiquitin to the substrate protein. We describe in this chapter the methods for the characterization of the expression profile of mRNA and protein of invariant members of SCF-complex and for the definition of SCF-complex activity. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 3 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Professor > Associate Professor | 1 | 33% |
Unknown | 2 | 67% |
Readers by discipline | Count | As % |
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Immunology and Microbiology | 1 | 33% |
Unknown | 2 | 67% |