Chapter title |
Label-Free Direct Detection of MiRNAs with Poly-Silicon Nanowire Biosensors
|
---|---|
Chapter number | 20 |
Book title |
MicroRNA Detection and Target Identification
|
Published in |
Methods in molecular biology, April 2017
|
DOI | 10.1007/978-1-4939-6866-4_20 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6864-0, 978-1-4939-6866-4
|
Authors |
Jing He, Jianjun Zhu, Bin Jiang, Yulan Zhao, He, Jing, Zhu, Jianjun, Jiang, Bin, Zhao, Yulan |
Editors |
Tamas Dalmay |
Abstract |
The diagnostic and prognostic value of microRNAs (miRNAs) in diseases becomes promising. Owing to fast response and high sensitivity, silicon nanowire (SiNW) biosensor has been considered a potential tool for miRNAs detection. Here, we describe a booming method to detect miRNAs with poly-silicon nanowire biosensors. Standard and real miRNA samples are applied in this study. The results show a limitation of 1 fM in the detection of standard miRNA sample with our poly-nanowire devices. Meanwhile, one-base mismatched sequence could be distinguished. Furthermore, these poly-SiNW arrays can detect snRNA U6 in total RNA samples extracted from HepG2 cells with a detection limitation of 0.2 μg/mL. |
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Unknown | 8 | 27% |