Chapter title |
Genome-Wide Comparison of Next-Generation Sequencing and qPCR Platforms for microRNA Profiling in Serum
|
---|---|
Chapter number | 3 |
Book title |
MicroRNA Detection and Target Identification
|
Published in |
Methods in molecular biology, April 2017
|
DOI | 10.1007/978-1-4939-6866-4_3 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6864-0, 978-1-4939-6866-4
|
Authors |
Thorarinn Blondal, Maurizia Rossana Brunetto, Daniela Cavallone, Martin Mikkelsen, Michael Thorsen, Yuan Mang, Hazel Pinheiro, Ferruccio Bonino, Peter Mouritzen, Blondal, Thorarinn, Brunetto, Maurizia Rossana, Cavallone, Daniela, Mikkelsen, Martin, Thorsen, Michael, Mang, Yuan, Pinheiro, Hazel, Bonino, Ferruccio, Mouritzen, Peter |
Editors |
Tamas Dalmay |
Abstract |
This study compares next-generation sequencing (NGS) technologies that have been optimized specifically for biofluid samples, with more established qPCR-based methods for profiling microRNAs in biofluids. The same patient serum samples were analyzed by NGS and qPCR, and differences in the serum microRNA profile between HBV and HCV infected patients were investigated. While there was overall good agreement between NGS and qPCR, there were some differences between the platforms, highlighting the importance of validation. |
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