Chapter title |
Single-Molecule Imaging of Signal Transduction via GPI-Anchored Receptors
|
---|---|
Chapter number | 19 |
Book title |
Lipid Signaling Protocols
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3170-5_19 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3169-9, 978-1-4939-3170-5
|
Authors |
Kenichi G. N. Suzuki, Kota Suzuki, Masamitsu Sakuma, Satoshi Hori, Tetsuya Nakazawa, Miki Nagao, Masao Yonemura, Masaaki Hirayama, Ryoji Kanno |
Abstract |
Lipid rafts have been drawing extensive attention as a signaling platform. To investigate molecular interactions in lipid rafts, we often need to observe molecules in the plasma membranes of living cells because chemical fixation and subsequent immunostaining with divalent or multivalent antibodies may change the location of the target molecules. In this chapter, we describe how to examine dynamics of raft-associated glycosylphosphatidylinositol (GPI)-anchored receptors and interactions of the receptors with downstream signaling molecules by single-particle tracking or single-molecule imaging techniques. |
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Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 1 | 9% |
Other | 1 | 9% |
Student > Doctoral Student | 1 | 9% |
Unknown | 3 | 27% |
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Physics and Astronomy | 1 | 9% |
Other | 2 | 18% |
Unknown | 2 | 18% |