| Chapter title |
Lentiviral gene transfer method to study integrin function in T lymphocytes.
|
|---|---|
| Chapter number | 4 |
| Book title |
Integrin and Cell Adhesion Molecules
|
| Published in |
Methods in molecular biology, June 2011
|
| DOI | 10.1007/978-1-61779-166-6_4 |
| Pubmed ID | |
| Book ISBNs |
978-1-61779-165-9, 978-1-61779-166-6
|
| Authors |
Daliya Banerjee, Motomu Shimaoka, Banerjee, Daliya, Shimaoka, Motomu |
| Editors |
Motomu Shimaoka |
| Abstract |
Integrins play critical roles in adhesion and migration of T cells during an immune response and inflammation. It is of great importance to understand the molecular pathways that regulate integrin function in T cells. Lentiviral vector-based gene transfer method has emerged in the past decade as an efficient means of transferring genes into both resting and activated hard-to-transfect cells, including T cells to knockdown gene expression. Therefore, this technology could be utilized effectively to study different aspects of integrin function or even to perform genome-wide RNAi screens to look globally for regulators of integrin function in T cells. In this chapter, we provide the simplest protocol to infect activated CD4(+) human T cells with high efficiency. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 5 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 2 | 40% |
| Other | 1 | 20% |
| Student > Doctoral Student | 1 | 20% |
| Unknown | 1 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 2 | 40% |
| Immunology and Microbiology | 2 | 40% |
| Unknown | 1 | 20% |