Chapter title |
Growth rates of a human colon adenocarcinoma cell line are regulated by the milk protein alpha-lactalbumin.
|
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Chapter number | 14 |
Book title |
Bioactive Components of Human Milk
|
Published in |
Advances in experimental medicine and biology, January 2001
|
DOI | 10.1007/978-1-4615-1371-1_14 |
Pubmed ID | |
Book ISBNs |
978-1-4613-5521-2, 978-1-4615-1371-1
|
Authors |
Sternhagen, L G, Allen, J C, Lisa G. Sternhagen, Jonathan C. Allen, Sternhagen, Lisa G., Allen, Jonathan C. |
Abstract |
The whey protein alpha-lactalbumin, derived from human milk, has been shown to inhibit proliferation of mammary epithelial cells and rat kidney cells. We have shown that bovine alpha-lactalbumin also has antiproliferative effects in human colon adenocarcinoma cell lines. During a 5-day dose-dependent growth study, bovine alpha-lactalbumin was added to Caco-2 or HT-29 monolayers in amounts from 5 to 35 microg/mL. Low concentrations of alpha-lactalbumin (10-25 microg/mL) stimulated growth during the first 3 to 4 days. After growing for 4 days, proliferation ceased and viable cell numbers decreased dramatically in the alpha-lactalbumin-treated cultures, suggesting a delayed initiation of apoptosis. This experiment demonstrates the acute bioactive effects of small concentrations of alpha-lactalbumin, compared with the high concentrations of other proteins in the media. These results suggest that alpha-lactalbumin in milk may promote health by inhibiting growth of potential cancer cells. Further studies will identify the role of calcium in the bioactivity of alpha-lactalbumin. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Brazil | 1 | 4% |
Unknown | 23 | 96% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 4 | 17% |
Student > Bachelor | 3 | 13% |
Professor > Associate Professor | 3 | 13% |
Researcher | 3 | 13% |
Student > Doctoral Student | 2 | 8% |
Other | 4 | 17% |
Unknown | 5 | 21% |
Readers by discipline | Count | As % |
---|---|---|
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Medicine and Dentistry | 4 | 17% |
Biochemistry, Genetics and Molecular Biology | 2 | 8% |
Immunology and Microbiology | 1 | 4% |
Nursing and Health Professions | 1 | 4% |
Other | 2 | 8% |
Unknown | 6 | 25% |