Chapter title |
Selective Reaction Monitoring Mass Spectrometry for Quantitation of Glycolytic Enzymes in Postmortem Brain Samples
|
---|---|
Chapter number | 16 |
Book title |
Proteomic Methods in Neuropsychiatric Research
|
Published in |
Advances in experimental medicine and biology, March 2017
|
DOI | 10.1007/978-3-319-52479-5_16 |
Pubmed ID | |
Book ISBNs |
978-3-31-952478-8, 978-3-31-952479-5
|
Authors |
Guilherme Lanfredi, Guiherme Reis-de-Oliveira, Veronica M. Saia-Cereda, Paul C. Guest, Daniel Martins-de-Souza, Vitor M. Faça |
Editors |
Paul C. Guest |
Abstract |
Patients with psychiatric disorders exhibit dysfunctions in peripheral and central metabolism. This may be a root cause of impaired neuronal function, manifested as changes in mood, behavior, and cognitive capabilities in patients suffering with these conditions. Here we describe a selective reaction monitoring mass spectrometry (SRM-MS)-based targeted proteomic protocol for precise simultaneous quantitation of three glycolytic enzymes in postmortem brain tissue extracts. The SRM-MS approach has several advantages in terms of sensitivity, reproducibility, and reduced sample consumption, compared to traditional MS methods. |
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