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Coronaviruses

Overview of attention for book
Cover of 'Coronaviruses'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Coronaviruses: An Overview of Their Replication and Pathogenesis
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    Chapter 2 Identification of a novel coronavirus from Guinea fowl using metagenomics.
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    Chapter 3 Serological diagnosis of feline coronavirus infection by immunochromatographic test.
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    Chapter 4 Estimation of Evolutionary Dynamics and Selection Pressure in Coronaviruses
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    Chapter 5 The Preparation of Chicken Tracheal Organ Cultures for Virus Isolation, Propagation, and Titration
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    Chapter 6 The Preparation of Chicken Kidney Cell Cultures for Virus Propagation
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    Chapter 7 Isolation and Propagation of Coronaviruses in Embryonated Eggs
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    Chapter 8 Characterization of Human Coronaviruses on Well-Differentiated Human Airway Epithelial Cell Cultures
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    Chapter 9 Quantification of Infectious Bronchitis Coronavirus by Titration In Vitro and In Ovo
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    Chapter 10 Purification of Coronavirus Virions for Cryo-EM and Proteomic Analysis.
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    Chapter 11 Partial Purification of IBV and Subsequent Isolation of Viral RNA for Next-Generation Sequencing
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    Chapter 12 Transient Dominant Selection for the Modification and Generation of Recombinant Infectious Bronchitis Coronaviruses
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    Chapter 13 Engineering Infectious cDNAs of Coronavirus as Bacterial Artificial Chromosomes
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    Chapter 14 Protein Histochemistry Using Coronaviral Spike Proteins: Studying Binding Profiles and Sialic Acid Requirements for Attachment to Tissues
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    Chapter 15 Identification of protein receptors for coronaviruses by mass spectrometry.
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    Chapter 16 Single Particle Tracking Assay to Study Coronavirus Membrane Fusion
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    Chapter 17 Studying coronavirus-host protein interactions.
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    Chapter 18 A field-proven yeast two-hybrid protocol used to identify coronavirus-host protein-protein interactions.
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    Chapter 19 Investigation of the Functional Roles of Host Cell Proteins Involved in Coronavirus Infection Using Highly Specific and Scalable RNA Interference (RNAi) Approach
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    Chapter 20 Transcriptome analysis of feline infectious peritonitis virus infection.
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    Chapter 21 Quantification of Interferon Signaling in Avian Cells
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    Chapter 22 Studying the Dynamics of Coronavirus Replicative Structures
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    Chapter 23 Preparation of Cultured Cells Using High-Pressure Freezing and Freeze Substitution for Subsequent 2D or 3D Visualization in the Transmission Electron Microscope
Attention for Chapter 19: Investigation of the Functional Roles of Host Cell Proteins Involved in Coronavirus Infection Using Highly Specific and Scalable RNA Interference (RNAi) Approach
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Chapter title
Investigation of the Functional Roles of Host Cell Proteins Involved in Coronavirus Infection Using Highly Specific and Scalable RNA Interference (RNAi) Approach
Chapter number 19
Book title
Coronaviruses
Published in
Methods in molecular biology, February 2015
DOI 10.1007/978-1-4939-2438-7_19
Pubmed ID
Book ISBNs
978-1-4939-2437-0, 978-1-4939-2438-7
Authors

Millet, Jean Kaoru, Nal, Béatrice, Jean Kaoru Millet, Béatrice Nal

Editors

Helena Jane Maier, Erica Bickerton, Paul Britton

Abstract

Since its identification in the 1990s, the RNA interference (RNAi) pathway has proven extremely useful in elucidating the function of proteins in the context of cells and even whole organisms. In particular, this sequence-specific and powerful loss-of-function approach has greatly simplified the study of the role of host cell factors implicated in the life cycle of viruses. Here, we detail the RNAi method we have developed and used to specifically knock down the expression of ezrin, an actin binding protein that was identified by yeast two-hybrid screening to interact with the Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV) spike (S) protein. This method was used to study the role of ezrin, specifically during the entry stage of SARS-CoV infection.

X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 23 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 23 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 6 26%
Other 2 9%
Librarian 2 9%
Student > Bachelor 2 9%
Student > Ph. D. Student 2 9%
Other 5 22%
Unknown 4 17%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 6 26%
Agricultural and Biological Sciences 3 13%
Veterinary Science and Veterinary Medicine 2 9%
Immunology and Microbiology 2 9%
Nursing and Health Professions 2 9%
Other 2 9%
Unknown 6 26%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 March 2015.
All research outputs
#15,325,572
of 22,793,427 outputs
Outputs from Methods in molecular biology
#5,328
of 13,110 outputs
Outputs of similar age
#213,399
of 357,845 outputs
Outputs of similar age from Methods in molecular biology
#322
of 975 outputs
Altmetric has tracked 22,793,427 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,110 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 45th percentile – i.e., 45% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 357,845 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 31st percentile – i.e., 31% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 975 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 58% of its contemporaries.