Chapter title |
Analysis of Meiotic Chromosome-Associated Protein Dynamics Using Conditional Expression in Budding Yeast
|
---|---|
Chapter number | 8 |
Book title |
Meiosis
|
Published in |
Methods in molecular biology, March 2017
|
DOI | 10.1007/978-1-4939-6340-9_8 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6338-6, 978-1-4939-6340-9
|
Authors |
MacQueen, Amy J., Rockmill, Beth, Amy J. MacQueen, Beth Rockmill |
Abstract |
The visualization of meiotic chromosomes and their associated protein structures in both wild-type and mutant cells adds valuable insight into the molecular pathways that underlie reproductive cell formation. Here we describe basic methodology for visualizing meiotic chromosomes in a long-standing model organism for investigating the molecular and cell biology of meiosis, the budding yeast, S. cerevisiae. This chapter furthermore highlights a variety of conditional expression regimes that can be used to understand the dynamics and/or developmental constraints of chromosomal protein structures; such dynamic aspects of the macromolecular structures that mediate meiotic chromosome biology are typically not obvious from standard protein visualization experiments. |
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