Chapter title |
Immunofluorescence Microscopy for the Detection of Surface Antigens in Methicillin-Resistant Staphylococcus aureus (MRSA).
|
---|---|
Chapter number | 4 |
Book title |
Methicillin-Resistant Staphylococcus Aureus (MRSA) Protocols
|
Published in |
Methods in molecular biology, January 2014
|
DOI | 10.1007/978-1-62703-664-1_4 |
Pubmed ID | |
Book ISBNs |
978-1-62703-663-4, 978-1-62703-664-1
|
Authors |
Yekaterina Timofeyeva, Ingrid L Scully, Annaliesa S Anderson, Ingrid L. Scully, Annaliesa S. Anderson, Timofeyeva, Yekaterina, Scully, Ingrid L., Anderson, Annaliesa S. |
Abstract |
Immunofluorescence microscopy is a widely used laboratory method which allows detection and visualization of specific antigens. The method employs the specificity of antibodies to deliver fluorophore to a specific target and then visualize it with a microscope. The power of the technique is that it requires relatively little manipulation and relatively few bacterial cells, enabling the detection of antigen expression where other methods cannot, such as during an actual infection in an animal. Here, we apply the method to follow antigen expression on the surface of MRSA cells over time in in vivo infection models. |
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