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Methicillin-Resistant Staphylococcus Aureus (MRSA) Protocols

Overview of attention for book
Cover of 'Methicillin-Resistant Staphylococcus Aureus (MRSA) Protocols'

Table of Contents

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    Book Overview
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    Chapter 1 Clinical, Epidemiologic, and Laboratory Aspects of Methicillin-Resistant Staphylococcus aureus Infections.
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    Chapter 2 Community-Associated Methicillin-Resistant Staphylococcus aureus Case Studies.
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    Chapter 3 Rapid methods for detection of MRSA in clinical specimens.
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    Chapter 4 Immunofluorescence Microscopy for the Detection of Surface Antigens in Methicillin-Resistant Staphylococcus aureus (MRSA).
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    Chapter 5 Internal Transcribed Spacer (ITS)-PCR Identification of MRSA.
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    Chapter 6 Pulsed-Field Gel Electrophoresis Typing of Staphylococcus aureus Isolates
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    Chapter 7 Multilocus Sequence Typing (MLST) of Staphylococcus aureus
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    Chapter 8 Staphylococcal Cassette Chromosome mec (SCCmec) Analysis of MRSA.
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    Chapter 9 Genetic Interruption of Target Genes for Investigation of Virulence Factors
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    Chapter 10 Molecular Analysis of Staphylococcal Superantigens
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    Chapter 11 Investigation of Staphylococcus aureus Adhesion and Invasion of Host Cells
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    Chapter 12 Investigation of Biofilm Formation in Clinical Isolates of Staphylococcus aureus.
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    Chapter 13 Transcriptomic Analysis of Staphylococcus aureus Using Microarray and Advanced Next-Generation RNA-seq Technologies.
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    Chapter 14 Proteomic Approach to Investigate Pathogenicity and Metabolism of Methicillin-Resistant Staphylococcus aureus.
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    Chapter 15 Metabolomic Investigation of Methicillin-Resistant Staphylococcus aureus
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    Chapter 16 Treatment of Infections Due to Resistant Staphylococcus aureus.
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    Chapter 17 Anti-infective Drug Development for MRSA.
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    Chapter 18 Animal Models in Drug Development for MRSA.
Attention for Chapter 4: Immunofluorescence Microscopy for the Detection of Surface Antigens in Methicillin-Resistant Staphylococcus aureus (MRSA).
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Chapter title
Immunofluorescence Microscopy for the Detection of Surface Antigens in Methicillin-Resistant Staphylococcus aureus (MRSA).
Chapter number 4
Book title
Methicillin-Resistant Staphylococcus Aureus (MRSA) Protocols
Published in
Methods in molecular biology, January 2014
DOI 10.1007/978-1-62703-664-1_4
Pubmed ID
Book ISBNs
978-1-62703-663-4, 978-1-62703-664-1
Authors

Yekaterina Timofeyeva, Ingrid L Scully, Annaliesa S Anderson, Ingrid L. Scully, Annaliesa S. Anderson, Timofeyeva, Yekaterina, Scully, Ingrid L., Anderson, Annaliesa S.

Abstract

Immunofluorescence microscopy is a widely used laboratory method which allows detection and visualization of specific antigens. The method employs the specificity of antibodies to deliver fluorophore to a specific target and then visualize it with a microscope. The power of the technique is that it requires relatively little manipulation and relatively few bacterial cells, enabling the detection of antigen expression where other methods cannot, such as during an actual infection in an animal. Here, we apply the method to follow antigen expression on the surface of MRSA cells over time in in vivo infection models.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 26 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Portugal 1 4%
Switzerland 1 4%
Unknown 24 92%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 4 15%
Student > Ph. D. Student 4 15%
Student > Master 3 12%
Researcher 3 12%
Other 2 8%
Other 4 15%
Unknown 6 23%
Readers by discipline Count As %
Agricultural and Biological Sciences 9 35%
Medicine and Dentistry 6 23%
Immunology and Microbiology 2 8%
Biochemistry, Genetics and Molecular Biology 1 4%
Environmental Science 1 4%
Other 1 4%
Unknown 6 23%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 October 2013.
All research outputs
#18,349,805
of 22,725,280 outputs
Outputs from Methods in molecular biology
#7,858
of 13,085 outputs
Outputs of similar age
#229,271
of 305,147 outputs
Outputs of similar age from Methods in molecular biology
#293
of 594 outputs
Altmetric has tracked 22,725,280 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,085 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 305,147 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 13th percentile – i.e., 13% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 594 others from the same source and published within six weeks on either side of this one. This one is in the 32nd percentile – i.e., 32% of its contemporaries scored the same or lower than it.