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Fluorescent Methods for Molecular Motors

Overview of attention for book
Cover of 'Fluorescent Methods for Molecular Motors'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Fluorescence and Labelling: How to Choose and What to Do
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    Chapter 2 Fluorescent Biosensors: Design and Application to Motor Proteins
  4. Altmetric Badge
    Chapter 3 Rapid Reaction Kinetic Techniques
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    Chapter 4 Fluorescence to study the ATPase mechanism of motor proteins.
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    Chapter 5 Use of pyrene-labelled actin to probe actin-Myosin interactions: kinetic and equilibrium studies.
  7. Altmetric Badge
    Chapter 6 Fluorescent Methods to Study Transcription Initiation and Transition into Elongation
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    Chapter 7 Single-molecule and single-particle imaging of molecular motors in vitro and in vivo.
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    Chapter 8 Fluorescence Methods in the Investigation of the DEAD-Box Helicase Mechanism
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    Chapter 9 Use of Fluorescent Techniques to Study the In Vitro Movement of Myosins
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    Chapter 10 Fluorescence tracking of motor proteins in vitro.
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    Chapter 11 Measuring Transport of Motor Cargos
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    Chapter 12 Measuring Two at the Same Time: Combining Magnetic Tweezers with Single-Molecule FRET
  14. Altmetric Badge
    Chapter 13 Using Fluorescence to Study Actomyosin in Yeasts
Attention for Chapter 5: Use of pyrene-labelled actin to probe actin-Myosin interactions: kinetic and equilibrium studies.
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1 tweeter

Citations

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Readers on

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Chapter title
Use of pyrene-labelled actin to probe actin-Myosin interactions: kinetic and equilibrium studies.
Chapter number 5
Book title
Fluorescent Methods for Molecular Motors
Published in
EXS, August 2014
DOI 10.1007/978-3-0348-0856-9_5
Pubmed ID
25095992
Book ISBNs
978-3-03-480855-2, 978-3-03-480856-9
Authors

Adamek N, Geeves MA, Nancy Adamek, Michael A. Geeves

Abstract

Studying the dynamics of the interaction between actin and myosin and how this is modulated by ATP and other nucleotides is fundamental to any understanding of myosin motor protein activity. The fluorescent label pyrene, covalently attached to actin (at Cys 374), has been one of the most useful optical probes to report myosin binding to actin. The unique spectral features of pyrene make it sensitive to changes in the microenvironment of the probe and allow to monitor processes such as conformational changes and protein-protein interactions. Here we describe how to make and use pyrene-labelled actin and describe a set of fluorescence stopped-flow measurements that allow the actin-myosin interaction to be explored at protein concentrations from μM to nM for many of the known myosin motors.

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Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 10 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 3 30%
Student > Bachelor 2 20%
Other 1 10%
Professor 1 10%
Lecturer > Senior Lecturer 1 10%
Other 0 0%
Unknown 2 20%
Readers by discipline Count As %
Agricultural and Biological Sciences 5 50%
Biochemistry, Genetics and Molecular Biology 2 20%
Medicine and Dentistry 1 10%
Unknown 2 20%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 October 2014.
All research outputs
#20,239,689
of 22,766,595 outputs
Outputs from EXS
#82
of 93 outputs
Outputs of similar age
#193,900
of 230,511 outputs
Outputs of similar age from EXS
#1
of 4 outputs
Altmetric has tracked 22,766,595 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 93 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 15.9. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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