Chapter title |
cDNA-AFLP-Based Transcript Profiling for Genome-Wide Expression Analysis of Jasmonate-Treated Plants and Plant Cultures
|
---|---|
Chapter number | 23 |
Book title |
Jasmonate Signaling
|
Published in |
Methods in molecular biology, April 2013
|
DOI | 10.1007/978-1-62703-414-2_23 |
Pubmed ID | |
Book ISBNs |
978-1-62703-413-5, 978-1-62703-414-2
|
Authors |
Colling, Janine, Pollier, Jacob, Makunga, Nokwanda P., Goossens, Alain, Janine Colling, Jacob Pollier, Nokwanda P. Makunga, Alain Goossens |
Editors |
Alain Goossens, Laurens Pauwels |
Abstract |
cDNA-AFLP is a commonly used, robust, and reproducible tool for genome-wide expression analysis in any species, without requirement of prior sequence knowledge. Quantitative expression data are generated by gel-based visualization of cDNA-AFLP fingerprints obtained by selective PCR amplification of subsets of restriction fragments from a double-stranded cDNA template. Differences in gene expression levels across the samples are reflected in different band intensities on the high-resolution polyacrylamide gels. The differentially expressed genes can be identified by direct sequencing of re-amplified cDNA-AFLP tags purified from the gels. The cDNA-AFLP technique is especially useful for profiling of transcriptional responses of jasmonate-treated plants or plant (tissue) cultures and the discovery of jasmonate-responsive genes. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 10 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 3 | 30% |
Student > Bachelor | 2 | 20% |
Other | 1 | 10% |
Lecturer | 1 | 10% |
Student > Doctoral Student | 1 | 10% |
Other | 2 | 20% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 3 | 30% |
Earth and Planetary Sciences | 1 | 10% |