Chapter title |
Detection of Food Allergens by Taqman Real-Time PCR Methodology
|
---|---|
Chapter number | 8 |
Book title |
Food Allergens
|
Published in |
Methods in molecular biology, March 2017
|
DOI | 10.1007/978-1-4939-6925-8_8 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6923-4, 978-1-4939-6925-8
|
Authors |
Aina García, Raquel Madrid, Teresa García, Rosario Martín, Isabel González |
Editors |
Jing Lin, Marcos Alcocer |
Abstract |
Real-time PCR (polymerase chain reaction) has shown to be a very effective technology for the detection of food allergens. The protocol described herein consists on a real-time PCR assay targeting the plant ITS (Internal Transcribed Spacer) region, using species-specific primers and hydrolysis probes (Taqman) dual labeled with a reporter fluorophore at the 5' end (6-carboxyfluorescein, FAM) and a quencher fluorophore at the 3' end (Blackberry, BBQ). The species-specific real-time PCR systems (primers/probe) described in this work allowed the detection of different nuts (peanut, hazelnut, pistachio, almond, cashew, macadamia, walnut and pecan), common allergens present in commercial food products, with a detection limit of 0.1 mg/kg. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 20 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Other | 3 | 15% |
Student > Bachelor | 3 | 15% |
Student > Master | 3 | 15% |
Researcher | 2 | 10% |
Professor | 1 | 5% |
Other | 3 | 15% |
Unknown | 5 | 25% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 6 | 30% |
Medicine and Dentistry | 3 | 15% |
Biochemistry, Genetics and Molecular Biology | 2 | 10% |
Computer Science | 2 | 10% |
Engineering | 1 | 5% |
Other | 0 | 0% |
Unknown | 6 | 30% |