Tandem Repeats in Genes, Proteins, and Disease
Humana Press
Chapter title |
PCR Amplification and Sequence Analysis of GC-Rich Sequences: Aristaless-Related Homeobox Example
|
---|---|
Chapter number | 8 |
Book title |
Tandem Repeats in Genes, Proteins, and Disease
|
Published in |
Methods in molecular biology, April 2013
|
DOI | 10.1007/978-1-62703-438-8_8 |
Pubmed ID | |
Book ISBNs |
978-1-62703-437-1, 978-1-62703-438-8
|
Authors |
Tan, May H., Gécz, Jozef, Shoubridge, Cheryl, May H. Tan, Jozef Gécz, Cheryl Shoubridge |
Abstract |
PCR amplification (followed by mutation scanning or direct sequencing) is a technique widely used in mutation detection and molecular studies of disease-causing genes, such as ARX. PCR amplification of high GC-rich regions encounters difficulties using conventional PCR procedures. Here, we present the strategies to amplify and sequence these GC-rich regions for the purposes of mutation screening and other molecular analyses. |
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