Tandem Repeats in Genes, Proteins, and Disease
Humana Press
Chapter title |
Challenges of “Sticky” Co-immunoprecipitation: Polyalanine Tract Protein–Protein Interactions
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Chapter number | 9 |
Book title |
Tandem Repeats in Genes, Proteins, and Disease
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Published in |
Methods in molecular biology, April 2013
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DOI | 10.1007/978-1-62703-438-8_9 |
Pubmed ID | |
Book ISBNs |
978-1-62703-437-1, 978-1-62703-438-8
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Authors |
Mattiske, T. R., Tan, May H., Gécz, Jozef, Shoubridge, Cheryl, T. R. Mattiske, May H. Tan, Jozef Gécz, Cheryl Shoubridge |
Abstract |
Co-immunoprecipitation (Co-IP) (followed by immunoblotting) is a technique widely used to characterize specific protein-protein interactions. Investigating interactions of proteins containing "sticky" polyalanine (PolyA) tracts encounters difficulties using conventional Co-IP procedures. Here, we present strategies to specifically capture proteins containing these difficult PolyA tracts, enabling subsequent robust detection of interacting proteins by Co-IP. |
Country | Count | As % |
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Unknown | 10 | 100% |
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 4 | 40% |
Student > Bachelor | 3 | 30% |
Professor > Associate Professor | 1 | 10% |
Professor | 1 | 10% |
Unknown | 1 | 10% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 4 | 40% |
Agricultural and Biological Sciences | 2 | 20% |
Neuroscience | 1 | 10% |
Unknown | 3 | 30% |