Chapter title |
A facile method for interfering with off-target silencing mediated by the sense strand.
|
---|---|
Chapter number | 18 |
Book title |
RNA Interference
|
Published in |
Methods in molecular biology, September 2014
|
DOI | 10.1007/978-1-4939-1538-5_18 |
Pubmed ID | |
Book ISBNs |
978-1-4939-1537-8, 978-1-4939-1538-5
|
Authors |
Anne Mobergslien, Mouldy Sioud, Mobergslien, Anne, Sioud, Mouldy |
Abstract |
Although siRNA duplexes are widely used for gene silencing, several unwanted effects such as activation of innate immunity and off-target gene silencing can limit their therapeutic use. Off-targeting can be identified for both the sense and antisense siRNA strands. Some avenues of obstructing the incorporation of the sense strand into the RNA-induced silencing complex (RISC) are currently being pursued. Herein, a biotin group at the 5'-end of the sense strand was used to inhibit its incorporation into the RISC complex. In contrast to chemical modifications, biotin is a naturally occurring compound and its presence in siRNA sequences will not induce side effects. |
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