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Matrix Metalloproteases

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Cover of 'Matrix Metalloproteases'

Table of Contents

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    Book Overview
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    Chapter 1 Expression and Purification of Matrix Metalloproteinases in Escherichia coli
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    Chapter 2 Expression and Purification of a Matrix Metalloprotease Transmembrane Domain in Escherichia coli
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    Chapter 3 Heterologous Expression of the Astacin Protease Meprin β in Pichia pastoris
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    Chapter 4 Structural Studies of Matrix Metalloproteinase by X-Ray Diffraction
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    Chapter 5 Mapping Lipid Bilayer Recognition Sites of Metalloproteinases and Other Prospective Peripheral Membrane Proteins
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    Chapter 6 Using Small Angle X-Ray Scattering (SAXS) to Characterize the Solution Conformation and Flexibility of Matrix Metalloproteinases (MMPs)
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    Chapter 7 Molecular Dynamics Studies of Matrix Metalloproteases
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    Chapter 8 Determining the Substrate Specificity of Matrix Metalloproteases using Fluorogenic Peptide Substrates
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    Chapter 9 Time-Resolved Analysis of Matrix Metalloproteinase Substrates in Complex Samples
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    Chapter 10 Identification of Protease Cleavage Sites by Charge-Based Enrichment of Protein N-Termini
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    Chapter 11 Mapping the Substrate Recognition Landscapes of Metalloproteases Using Comprehensive Mutagenesis
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    Chapter 12 Detection of Matrix Metalloproteinases by Zymography
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    Chapter 13 Imaging Matrix Metalloproteases in Spontaneous Colon Tumors: Validation by Correlation with Histopathology
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    Chapter 14 Virtual High-Throughput Screening for Matrix Metalloproteinase Inhibitors
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    Chapter 15 Computational Approaches to Matrix Metalloprotease Drug Design
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    Chapter 16 A Simple Adaptable Blood-Brain Barrier Cell Model for Screening Matrix Metalloproteinase Inhibitor Functionality
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    Chapter 17 Matrix Metalloproteases as Biomarkers of Disease
Attention for Chapter 12: Detection of Matrix Metalloproteinases by Zymography
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Chapter title
Detection of Matrix Metalloproteinases by Zymography
Chapter number 12
Book title
Matrix Metalloproteases
Published in
Methods in molecular biology, March 2017
DOI 10.1007/978-1-4939-6863-3_12
Pubmed ID
Book ISBNs
978-1-4939-6861-9, 978-1-4939-6863-3
Authors

Rajeev B. Tajhya, Rutvik S. Patel, Christine Beeton, Tajhya, Rajeev B., Patel, Rutvik S., Beeton, Christine

Editors

Charles A. Galea

Abstract

Matrix metalloproteinases (MMPs) represent more than 20 zinc-containing endopeptidases that cleave internal peptide bonds, leading to protein degradation. They play a critical role in many physiological cell functions, including tissue remodeling, embryogenesis, and angiogenesis. They are also involved in the pathogenesis of a vast array of diseases, including but not limited to systemic inflammation, various cancers, and cardiovascular, neurological, and autoimmune diseases. Here, we describe gel zymography to detect MMPs in cell and tissue samples and in cell culture supernatants.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 79 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 79 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 12 15%
Student > Ph. D. Student 11 14%
Student > Bachelor 11 14%
Student > Doctoral Student 7 9%
Lecturer 2 3%
Other 8 10%
Unknown 28 35%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 19 24%
Agricultural and Biological Sciences 9 11%
Medicine and Dentistry 7 9%
Pharmacology, Toxicology and Pharmaceutical Science 3 4%
Nursing and Health Professions 2 3%
Other 10 13%
Unknown 29 37%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 March 2017.
All research outputs
#18,339,860
of 22,711,242 outputs
Outputs from Methods in molecular biology
#7,851
of 13,079 outputs
Outputs of similar age
#234,431
of 307,634 outputs
Outputs of similar age from Methods in molecular biology
#187
of 313 outputs
Altmetric has tracked 22,711,242 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,079 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
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We're also able to compare this research output to 313 others from the same source and published within six weeks on either side of this one. This one is in the 22nd percentile – i.e., 22% of its contemporaries scored the same or lower than it.