Chapter title |
DNA-Directed Antibody Immobilization for Robust Protein Microarrays: Application to Single Particle Detection ‘DNA-Directed Antibody Immobilization
|
---|---|
Chapter number | 12 |
Book title |
Biosensors and Biodetection
|
Published in |
Methods in molecular biology, March 2017
|
DOI | 10.1007/978-1-4939-6848-0_12 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6846-6, 978-1-4939-6848-0
|
Authors |
Nese Lortlar Ünlü, Fulya Ekiz Kanik, Elif Seymour, John H. Connor, M. Selim Ünlü |
Editors |
Avraham Rasooly, Ben Prickril |
Abstract |
Protein microarrays are emerging tools which have become very powerful in multiplexed detection technologies. A variety of proteins can be immobilized on a sensor chip allowing for multiplexed diagnostics. Therefore, various types of analyte in a small volume of sample can be detected simultaneously. Protein immobilization is a crucial step for creating a robust and sensitive protein microarray-based detection system. In order to achieve a successful protein immobilization and preserve the activity of the proteins after immobilization, DNA-directed immobilization is a promising technique. Here, we present the design and the use of DNA-directed immobilized (DDI) antibodies in fabrication of robust protein microarrays. We focus on application of protein microarrays for capturing and detecting nanoparticles such as intact viruses. Experimental results on Single-particle interferometric reflectance imaging sensor (SP-IRIS) are used to validate the advantages of the DDI method. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 57 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 14 | 25% |
Researcher | 9 | 16% |
Student > Master | 7 | 12% |
Student > Bachelor | 4 | 7% |
Student > Doctoral Student | 3 | 5% |
Other | 7 | 12% |
Unknown | 13 | 23% |
Readers by discipline | Count | As % |
---|---|---|
Engineering | 13 | 23% |
Biochemistry, Genetics and Molecular Biology | 6 | 11% |
Chemistry | 5 | 9% |
Agricultural and Biological Sciences | 5 | 9% |
Chemical Engineering | 3 | 5% |
Other | 11 | 19% |
Unknown | 14 | 25% |