Chapter title |
Examining H2O2 Production in Arabidopsis Leaves Upon Challenge by Cytokinin
|
---|---|
Chapter number | 13 |
Book title |
Auxins and Cytokinins in Plant Biology
|
Published in |
Methods in molecular biology, March 2017
|
DOI | 10.1007/978-1-4939-6831-2_13 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6829-9, 978-1-4939-6831-2
|
Authors |
Shahjahan Shabbir Ahmed, Ihsan Ullah, Shazia Irfan, Nazeer Ahmed |
Editors |
Thomas Dandekar, Muhammad Naseem |
Abstract |
Plant cells respond to stresses and exogenous environmental stimuli. Production of altered levels of reactive oxygen species (ROS) is one of a typical cellular responses against such stimuli. These responses are detectable through various techniques including luminol-based bioassays. Luminol-based bioassays provide an excellent opportunity for detection of ROS in plant leaf tissue when challenged with some exogenous stimuli like phytohormones, cytokinins, auxins, ABA, etc. The luminol-based protocol in point aims at measuring changes in the H2O2 levels of Arabidopsis leaf discs when exposed to phytohormone cytokinins. However, utility of the luminol-based ROS determination protocol is quite wide. We specifically optimized this protocol to quantify the effect of cytokinins on ROS production by Arabidopsis leaves. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 4 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Professor > Associate Professor | 1 | 25% |
Student > Doctoral Student | 1 | 25% |
Unknown | 2 | 50% |
Readers by discipline | Count | As % |
---|---|---|
Environmental Science | 1 | 25% |
Biochemistry, Genetics and Molecular Biology | 1 | 25% |
Unknown | 2 | 50% |