Chapter 1 A Fluorescence-Based High-Throughput Screening Assay to Identify HIV-1 Inhibitors

Chapter 2 A Homogeneous Time-Resolved Fluorescence Assay to Identify Inhibitors of HIV-1 Fusion

Chapter 3 Identification of HIV-1 Reverse Transcriptase Inhibitors Using a Scintillation Proximity Assay

Chapter 4 Biochemical Screening Assays to Identify HIV-1 Integrase Inhibitors

Chapter 5 HIV-1 Genotyping of the Protease-Reverse Transcriptase and Integrase Genes to Detect Mutations That Confer Antiretroviral Resistance

Chapter 6 In Vitro Kinetic Profiling of Hepatitis C Virus NS3 Protease Inhibitors by Progress Curve Analysis

Chapter 7 A Novel Hepatitis C Virus NS5B Polymerase Assay of De Novo Initiated RNA Synthesis Directed from a Heteropolymeric RNA Template

Chapter 8 Selecting and Characterizing Drug-Resistant Hepatitis C Virus Replicon

Chapter 9 A Cellular Replicon-Based Phenotyping Assay to Determine Susceptibility of Hepatitis C Virus Clinical Isolates to NS3/4A Protease Inhibitors

Chapter 10 Expression and Purification of Hepatitis C Virus Protease from Clinical Samples

Chapter 11 Surface Plasmon Resonance as a Tool to Select Potent Drug Candidates for Hepatitis C Virus NS5B Polymerase

Chapter 12 Amplification and Sequencing of the Hepatitis C Virus NS3/4A Protease and the NS5B Polymerase Regions for Genotypic Resistance Detection of Clinical Isolates of Subtypes 1a and 1b

Chapter 14 In Vitro Phenotyping of Recombinant Hepatitis B Virus Containing the Polymerase/Reverse Transcriptase Gene from Clinical Isolates

Chapter 15 Cell-Based Antiviral Assays for Screening and Profiling Inhibitors Against Dengue Virus

Chapter 16 A Duplex Real-Time RT-PCR Assay for Profiling Inhibitors of Four Dengue Serotypes

Chapter 17 High-Throughput Screening Using Dengue Virus Reporter Genomes

Chapter 18 Fluorimetric and HPLC-Based Dengue Virus Protease Assays Using a FRET Substrate

Chapter 19 Expression and Purification of Dengue Virus NS5 Polymerase and Development of a High-Throughput Enzymatic Assay for Screening Inhibitors of Dengue Polymerase

Chapter 20 Detection and Quantification of Flavivirus NS5 Methyl-Transferase Activities

Chapter 21 Testing Antiviral Compounds in a Dengue Mouse Model

Chapter 22 Construction of Self-Replicating Subgenomic West Nile Virus Replicons for Screening Antiviral Compounds

Chapter 23 Methods for Screening and Profiling Inhibitors of Herpes Simplex Viruses

Chapter 24 In Vivo Evaluation of Antiviral Efficacy Against Genital Herpes Using Mouse and Guinea Pig Models

Chapter 25 A Fluorescence-Based High-Throughput Screening Assay for Identifying Human Cytomegalovirus Inhibitors

Chapter 26 A Fluorescence-Based High-Throughput Antiviral Compound Screening Assay Against Respiratory Syncytial Virus

Chapter 27 Screening and Evaluation of Anti-respiratory Syncytial Virus Compounds in Cultured Cells

Chapter 28 Evaluation of Antiviral Efficacy Against Human Respiratory Syncytial Virus Using Cotton Rat and Mouse Models

Chapter 29 In Vivo Evaluation of Antiviral Compounds on Respiratory Syncytial Virus Using a Juvenile Vervet Monkey (Chlorocebus pygerythrus) Infection Model

Chapter 30 Methods to Determine Mechanism of Action of Anti-influenza Inhibitors

Chapter 31 Methods for Evaluation of Antiviral Efficacy Against Influenza Virus Infections in Animal Models

Chapter 32 Development of a High-Throughput Antiviral Assay for Screening Inhibitors of Chikungunya Virus and Generation of Drug-Resistant Mutations in Cultured Cells

Chapter 33 A Mouse Model of Chikungunya Virus with Utility in Antiviral Studies