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Recombinant Protein Expression in Mammalian Cells

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Recombinant Protein Expression in Mammalian Cells
Springer US

Table of Contents

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    Book Overview
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    Chapter 1 PEI-Mediated Transient Gene Expression in CHO Cells
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    Chapter 2 Transient Co-expression of Membrane Protein Complexes in Mammalian Cells
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    Chapter 3 The Use of Baculovirus-Mediated Gene Expression in Mammalian Cells for Recombinant Protein Production
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    Chapter 4 Production and Purification of Adeno-Associated Viral Vectors (AAVs) Using Orbitally Shaken HEK293 Cells
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    Chapter 5 Protein Expression via Transient Transfection of Mammalian Cells in a WAVE Bioreactor
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    Chapter 6 Small-Scale Cultivation and Transfection of ExpiCHO and HEK293E Cells in Single-Use Orbitally Shaken Bioreactors
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    Chapter 7 Recombinant Protein Production from Stable CHO Cell Pools
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    Chapter 8 A Versatile Method for Inducible Protein Production in 293 Cells Using the PiggyBac Transposon System
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    Chapter 9 Recombinant CHO Cell Pool Generation Using PiggyBac Transposon System
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    Chapter 10 Stable Expression by Lentiviral Transduction of Cells
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    Chapter 11 Expression of Multispecific Antibodies
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    Chapter 12 Expression of Bispecific Antibodies Using Cellular Display
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    Chapter 13 Generation of Monoclonal Chinese Hamster Ovary Cell Lines Using DISPENCELL-S3
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    Chapter 14 Enhancing Cell Line Stability by CRISPR/Cas9-Mediated Site-Specific Integration Based on Histone Modifications
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    Chapter 15 Bench-Scale Stirred-Tank Bioreactor for Recombinant Protein Production in Chinese Hamster Ovary (CHO) Cells in Suspension
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    Chapter 16 Application of the CRISPR/Cas9 Gene Editing Method for Modulating Antibody Fucosylation in CHO Cells
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    Chapter 17 Development of Responsive Promoters and their Utilization for Stable CHO Sensor Cell Lines
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    Chapter 18 CRISPR Deletion of miR-27 Impacts Recombinant Protein Production in CHO Cells
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    Chapter 19 High-Throughput Protein Expression Screening of Cell-Surface Protein Ectodomains
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    Chapter 20 Genome-Wide High-Throughput RNAi Screening for Identification of Genes Involved in Protein Production
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    Chapter 21 Purification and Quality Control of Recombinant Proteins Expressed in Mammalian Cells: A Practical Review
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Title
Recombinant Protein Expression in Mammalian Cells
Published by
Springer US, June 2024
DOI 10.1007/978-1-0716-3878-1
ISBNs
978-1-07-163877-4, 978-1-07-163878-1
Editors

Hacker, David L.

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