Mitochondrial regulation

Chapter 1 Isolation of Crude Mitochondrial Fraction from Cells

Chapter 2 Isolation of Mitochondria from Liver and Extraction of Total RNA and Protein: Analyses of MicroRNA and Protein Expressions.

Chapter 3 PCR based determination of mitochondrial DNA copy number in multiple species.

Chapter 4 NAD + Content and Its Role in Mitochondria

Chapter 5 Measuring PGC-1α and Its Acetylation Status in Mouse Primary Myotubes.

Chapter 6 Measurement of Mitochondrial Oxygen Consumption Rates in Mouse Primary Neurons and Astrocytes

Chapter 7 Determination of Oxidative Phosphorylation Complexes Activities

Chapter 8 Histoenzymatic Methods for Visualization of the Activity of Individual Mitochondrial Respiratory Chain Complexes in the Muscle Biopsies from Patients with Mitochondrial Defects

Chapter 9 A method aimed at assessing the functional consequences of the supramolecular organization of the respiratory electron transfer chain by time-resolved studies.

Chapter 10 BN-PAGE-Based Approach to Study Thyroid Hormones and Mitochondrial Function

Chapter 11 Detection of UCP1 Protein and Measurements of Dependent GDP-Sensitive Proton Leak in Non-phosphorylating Thymus Mitochondria

Chapter 12 Exploring Liver Mitochondrial Function by 13 C-Stable Isotope Breath Tests: Implications in Clinical Biochemistry

Chapter 13 Following Mitochondria Dynamism: Confocal Analysis of the Organelle Morphology

Chapter 14 Analysis of Pro-apoptotic Protein Trafficking to and from Mitochondria

Chapter 15 Mitophagy and Mitochondrial Balance