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Mitochondrial regulation

Overview of attention for book
Cover of 'Mitochondrial regulation'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Isolation of Crude Mitochondrial Fraction from Cells
  3. Altmetric Badge
    Chapter 2 Isolation of Mitochondria from Liver and Extraction of Total RNA and Protein: Analyses of MicroRNA and Protein Expressions.
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    Chapter 3 PCR based determination of mitochondrial DNA copy number in multiple species.
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    Chapter 4 NAD + Content and Its Role in Mitochondria
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    Chapter 5 Measuring PGC-1α and Its Acetylation Status in Mouse Primary Myotubes.
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    Chapter 6 Measurement of Mitochondrial Oxygen Consumption Rates in Mouse Primary Neurons and Astrocytes
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    Chapter 7 Determination of Oxidative Phosphorylation Complexes Activities
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    Chapter 8 Histoenzymatic Methods for Visualization of the Activity of Individual Mitochondrial Respiratory Chain Complexes in the Muscle Biopsies from Patients with Mitochondrial Defects
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    Chapter 9 A method aimed at assessing the functional consequences of the supramolecular organization of the respiratory electron transfer chain by time-resolved studies.
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    Chapter 10 BN-PAGE-Based Approach to Study Thyroid Hormones and Mitochondrial Function
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    Chapter 11 Detection of UCP1 Protein and Measurements of Dependent GDP-Sensitive Proton Leak in Non-phosphorylating Thymus Mitochondria
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    Chapter 12 Exploring Liver Mitochondrial Function by 13 C-Stable Isotope Breath Tests: Implications in Clinical Biochemistry
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    Chapter 13 Following Mitochondria Dynamism: Confocal Analysis of the Organelle Morphology
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    Chapter 14 Analysis of Pro-apoptotic Protein Trafficking to and from Mitochondria
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    Chapter 15 Mitophagy and Mitochondrial Balance
Attention for Chapter 2: Isolation of Mitochondria from Liver and Extraction of Total RNA and Protein: Analyses of MicroRNA and Protein Expressions.
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Chapter title
Isolation of Mitochondria from Liver and Extraction of Total RNA and Protein: Analyses of MicroRNA and Protein Expressions.
Chapter number 2
Book title
Mitochondrial Regulation
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-1875-1_2
Pubmed ID
25308484
Book ISBNs
978-1-4939-1874-4, 978-1-4939-1875-1
Authors

Pedro M Borralho, Clifford J Steer, Cecília M P Rodrigues, Pedro M. Borralho, Clifford J. Steer, Cecília M. P. Rodrigues, Borralho, Pedro M., Steer, Clifford J., Rodrigues, Cecília M. P.

Abstract

Several studies have indicated the presence of microRNAs (miRNAs) within mitochondria, although the origin, as well as the biological function of these mitochondrially located miRNAs is largely unknown. The identification and significance of this subcellular localization is gaining increasing relevance to the pathogenesis of certain disease states. Here we describe the isolation of highly purified mitochondria from rat liver by differential centrifugation, followed by RNAse A treatment to eliminate contaminating RNA. The coupled extraction of total RNA and protein is a more efficient design for allowing the downstream evaluation of miRNA and protein expression in mitochondria.

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X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 18 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 6%
Unknown 17 94%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 5 28%
Researcher 4 22%
Professor > Associate Professor 2 11%
Student > Master 1 6%
Librarian 1 6%
Other 2 11%
Unknown 3 17%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 6 33%
Agricultural and Biological Sciences 4 22%
Medicine and Dentistry 3 17%
Pharmacology, Toxicology and Pharmaceutical Science 2 11%
Neuroscience 1 6%
Other 0 0%
Unknown 2 11%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 October 2014.
All research outputs
#20,239,689
of 22,766,595 outputs
Outputs from Methods in molecular biology
#9,865
of 13,090 outputs
Outputs of similar age
#295,623
of 352,893 outputs
Outputs of similar age from Methods in molecular biology
#635
of 996 outputs
Altmetric has tracked 22,766,595 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,090 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 352,893 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 996 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.

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