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Chapter title |
Whole-Genome Haplotyping of Single Sperm of Daphnia pulex (Crustacea, Anomopoda).
|
---|---|
Chapter number | 8 |
Book title |
Haplotyping
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Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6750-6_8 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6748-3, 978-1-4939-6750-6
|
Authors |
Xu, Sen, Young, Kim, Sen Xu, Kim Young |
Editors |
Irene Tiemann-Boege, Andrea Betancourt |
Abstract |
Sequencing the entire genome of single sperm cells can provide valuable information of the distribution of meiotic recombination events in eukaryotic genomes. Here, we provide a description of the experimental work flow for isolating single sperm cells from the microcrustacean Daphnia pulex using fluorescence-activated cell sorting. Moreover, we describe the application of a whole-genome amplification technique (i.e., Multiple Annealing and Looping Based Amplification Cycles method) to single sperm of Daphnia to generate enough DNA for library preparation of next-generation sequencing. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
As of 1 July 2024, you may notice a temporary increase in the numbers of X profiles with Unknown location. Click here to learn more.
As of 1 July 2024, you may notice a temporary increase in the numbers of X profiles with Unknown location. Click here to learn more.
Geographical breakdown
Country | Count | As % |
---|---|---|
Japan | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 6 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 4 | 67% |
Researcher | 1 | 17% |
Unknown | 1 | 17% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 3 | 50% |
Agricultural and Biological Sciences | 2 | 33% |
Unknown | 1 | 17% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 February 2017.
All research outputs
#15,440,760
of 22,950,943 outputs
Outputs from Methods in molecular biology
#5,372
of 13,138 outputs
Outputs of similar age
#256,997
of 420,989 outputs
Outputs of similar age from Methods in molecular biology
#466
of 1,074 outputs
Altmetric has tracked 22,950,943 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,138 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 44th percentile – i.e., 44% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 420,989 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 30th percentile – i.e., 30% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1,074 others from the same source and published within six weeks on either side of this one. This one is in the 40th percentile – i.e., 40% of its contemporaries scored the same or lower than it.