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KRAS

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Cover of 'KRAS'

Table of Contents

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    Book Overview
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    Chapter 1 Progress in Targeting KRAS Directly
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    Chapter 2 The Abundance of KRAS and RAS Gene Mutations in Cancer
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    Chapter 3 Production of Isotopically Labeled KRAS4b
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    Chapter 4 Nucleotide Exchange on RAS Proteins Using Hydrolysable and Non-hydrolysable Nucleotides
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    Chapter 5 Crystallographic Studies of KRAS in Complex with Small Molecules and RAS-Binding Proteins
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    Chapter 6 Considerations Around Structure-Based Drug Discovery for KRAS Using DOCK
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    Chapter 7 Measurement of KRAS-GTPase Activity
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    Chapter 8 Affinity Measurement of Non-covalent Interactions of the Covalent KRAS G12C GDP Inhibitor MRTX849 to RAS Isoforms Using Surface Plasmon Resonance
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    Chapter 9 1D and 2D NMR for KRAS:Ligand Binding
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    Chapter 10 Luminescence-Based Techniques for KRAS Thermal Stability Monitoring
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    Chapter 11 MALDI-TOF Mass Spectrometry-Based Assay for Measuring Covalent Target Engagement of KRAS G12C Inhibitors
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    Chapter 12 KRAS4b:RAF-1 Homogenous Time-Resolved Fluorescence Resonance Energy Transfer Assay for Drug Discovery
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    Chapter 13 Biophysical Characterization of RAS-SOS Complexes by Native Mass Spectrometry
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    Chapter 14 Profiling Complex RAS-Effector Interactions Using NMR Spectroscopy
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    Chapter 15 Probing RAS Function Using Monobody and NanoBiT Technologies
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    Chapter 16 Analysis of the Guanine Nucleotide-Bound State of KRAS by Ion-Pair Reversed-Phase High-Performance Liquid Chromatography
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    Chapter 17 Real-Time Monitoring of RAS Activity Using In Vitro and In-Cell NMR Spectroscopy
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    Chapter 18 Studying RAS Interactions in Live Cells with BRET
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    Chapter 19 FLIM-FRET Protein-Protein Interaction Assay
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    Chapter 20 High-Throughput Cell-Based Screening of Small Molecule KRAS Signaling Inhibitors Using a Homogeneous Time-Resolved Fluorescence (HTRF) Assay
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    Chapter 21 A Method to Conditionally Measure Target Engagement at Intracellular RAS and RAF Complexes
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    Chapter 22 FLAG-KRAS4B as a Model System for KRAS4B Proteoform and PTM Evaluation by Mass Spectrometry
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    Chapter 23 Creation of an Isogenic H/N/KRAS-Less Mouse Embryonic Fibroblast Cell Line Panel Derived from a Size-Sorted Diploid Clone
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    Chapter 24 Quality Control of an Isogenic H/N/KRAS-Less Mouse Embryonic Fibroblast Cell Line Panel
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    Chapter 25 A Facile Method to Append a Bio-ID Tag to Endogenous Mutant Kras Alleles
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Title
KRAS
Published by
Springer US, May 2024
DOI 10.1007/978-1-0716-3822-4
ISBNs
978-1-07-163821-7, 978-1-07-163822-4
Editors

Stephen, Andrew G., Esposito, Dominic

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