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Phage Engineering and Analysis

Overview of attention for book
Cover of 'Phage Engineering and Analysis'

Table of Contents

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    Book Overview
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    Chapter 1 Protocols for Building and Producing High Diversity Peptide Phage Display Libraries.
  3. Altmetric Badge
    Chapter 2 Generation of a Naïve Human scFv Phage Display Library and Panning Selection.
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    Chapter 3 Structure-Guided and Phage-Assisted Evolution of Therapeutic Antibodies to Reverse On-Target Point Mutation-Mediated Resistance.
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    Chapter 4 Combining a Base Deaminase Mutator with Phage-Assisted Evolution.
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    Chapter 5 Understanding the Structural Requirements of Peptide-Protein Interaction and Applications for Peptidomimetic Development.
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    Chapter 6 Contracting the Host Range of Bacteriophage T7 Using a Continuous Evolution System.
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    Chapter 7 Single-Cell Proteomics by Barcoded Phage-Displayed Screening via an Integrated Microfluidic Chip
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    Chapter 8 Targeted Genome Editing of Virulent Pseudomonas Phages Using CRISPR-Cas3
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    Chapter 9 Preparation of Bioconjugates of Chimeric M13 Phage and Gold Nanorods
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    Chapter 10 Liquid Glycan Array
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    Chapter 11 Electron Microscopy Methods for Phage-Based Study
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    Chapter 12 Visualization of Engineered M13 Phages Bound to Bacterial Targets by Transmission Electron Microscopy
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    Chapter 13 Purification of Single-Stranded RNA Bacteriophages and Host Receptors for Structural Determination Using Cryo-Electron Microscopy
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    Chapter 14 Obtaining Detailed Phage Transcriptomes Using ONT-Cappable-Seq.
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    Chapter 16 CRISPRpi: Inducing and Curing Prophage Using the CRISPR Interference
  17. Altmetric Badge
    Chapter 17 Isolation of Bacteriophages on Actinobacteria Hosts
Attention for Chapter 3: Structure-Guided and Phage-Assisted Evolution of Therapeutic Antibodies to Reverse On-Target Point Mutation-Mediated Resistance.
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Chapter title
Structure-Guided and Phage-Assisted Evolution of Therapeutic Antibodies to Reverse On-Target Point Mutation-Mediated Resistance.
Chapter number 3
Book title
Phage Engineering and Analysis
Published in
Methods in molecular biology, January 2024
DOI 10.1007/978-1-0716-3798-2_3
Pubmed ID
38526722
Book ISBNs
978-1-07-163797-5, 978-1-07-163798-2
Authors

Zhuang, Xinlei, Chen, Shuqing, Pan, Liqiang

Abstract

Resistance to therapeutic antibodies caused by on-target point mutations is a major obstacle in anticancer therapy, creating an "unmet clinical need." To tackle this problem, researchers are developing new generations of antibody drugs that can overcome the resistance mechanisms of existing agents. We have previously reported a structure-guided and phage-assisted evolution (SGAPAE) approach to evolve cetuximab, a therapeutic antibody, to effectively reverse the resistance driven by EGFRS492R or EGFRG465R mutations, without changing the binding epitope or compromising the antibody efficacy. In this protocol, we provide detailed instructions on how to use the SGAPAE approach to evolve cetuximab, which can also be applied to other therapeutic antibodies for reversing on-target point mutation-mediated resistance. The protocol consists of four steps: structure preparation, computational prediction, phage display library construction, and antibody candidate selection.

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 Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 March 2024.
All research outputs
#17,424,278
of 25,562,515 outputs
Outputs from Methods in molecular biology
#6,088
of 14,297 outputs
Outputs of similar age
#182,985
of 345,196 outputs
Outputs of similar age from Methods in molecular biology
#117
of 289 outputs
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So far Altmetric has tracked 14,297 research outputs from this source. They receive a mean Attention Score of 3.5. This one is in the 41st percentile – i.e., 41% of its peers scored the same or lower than it.
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We're also able to compare this research output to 289 others from the same source and published within six weeks on either side of this one. This one is in the 22nd percentile – i.e., 22% of its contemporaries scored the same or lower than it.

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