Chapter title |
Combining a Base Deaminase Mutator with Phage-Assisted Evolution.
|
---|---|
Chapter number | 4 |
Book title |
Phage Engineering and Analysis
|
Published in |
Methods in molecular biology, January 2024
|
DOI | 10.1007/978-1-0716-3798-2_4 |
Pubmed ID | |
Book ISBNs |
978-1-07-163797-5, 978-1-07-163798-2
|
Authors |
Ali, Maryam, Akel, Raneem, Botero, Maria J, Shin, Jumi A, Botero, Maria J., Shin, Jumi A. |
Abstract |
Phage-assisted evolution has emerged as a powerful technique for improving a protein's function by using mutagenesis and selective pressure. However, mutations typically occur throughout the host's genome and are not limited to the gene-of-interest (GOI): these undesirable genomic mutations can yield host cells that circumvent the system's selective pressure. Our system targets mutations specifically toward the GOI by combining T7 targeted mutagenesis and phage-assisted evolution. This system improves the structure and function of proteins by accumulating favorable mutations that can change its binding affinity, specificity, and activity. |
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