Chapter title |
Isolation of GMP Grade Human Hepatocytes from Remnant Liver Tissue of Living Donor Liver Transplantation
|
---|---|
Chapter number | 16 |
Book title |
Hepatocyte Transplantation
|
Published in |
Methods in molecular biology, November 2016
|
DOI | 10.1007/978-1-4939-6506-9_16 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6504-5, 978-1-4939-6506-9
|
Authors |
Shin Enosawa |
Editors |
Peggy Stock, Bruno Christ |
Abstract |
For the purpose of clinical research of hepatocyte transplantation, procedures for isolation, cryopreservation, thawing, and functional assessment of hepatocytes are described. Although demands for human hepatocytes are increasing in not only cell therapy but also drug development, it is highly difficult to obtain good lots of hepatocytes from human liver tissue. This chapter describes essential issues such as alleviation of warm ischemia, prevention of shear stress, optimization of cryopreservation, and functional assessment, along with securement of quality. All procedures described here are compliant with good manufacturing procedure (GMP) in cell processing facility, approved by the act on measures to ensure safety of regenerative medicine and ethical regulations in Japan. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 6 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 2 | 33% |
Researcher | 2 | 33% |
Student > Bachelor | 1 | 17% |
Lecturer | 1 | 17% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 2 | 33% |
Biochemistry, Genetics and Molecular Biology | 1 | 17% |
Philosophy | 1 | 17% |
Agricultural and Biological Sciences | 1 | 17% |
Nursing and Health Professions | 1 | 17% |
Other | 0 | 0% |