Trophoblasts
Springer US
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Attention Score in Context
| Chapter title |
Real-Time Assessment of Mitochondrial Function in Cytotrophoblast and Syncytialized Trophoblast Cells Using the Seahorse XFe24 Extracellular Flux Analyzer.
|
|---|---|
| Chapter number | 12 |
| Book title |
Trophoblasts
|
| Published in |
Methods in molecular biology, January 2024
|
| DOI | 10.1007/978-1-0716-3495-0_12 |
| Pubmed ID | |
| Book ISBNs |
978-1-07-163494-3, 978-1-07-163495-0
|
| Authors |
Walker, O'Llenecia S, May, Linda L, Raha, Sandeep, Walker, O’Llenecia S., May, Linda L. |
| Abstract |
Physiological processes utilize variable amounts of energy required for optimal growth, development, and survival. This energy is supplied by total intracellular adenosine triphosphate (ATP) and is mainly generated by mitochondrial oxidative phosphorylation and to a lesser extent via glycolysis. Here, we provide a detailed protocol for obtaining measurements of energy metabolism using the Seahorse XFe24 Extracellular Flux Analyzer. Specifically, this assay measures mitochondrial oxidative phosphorylation based on oxygen consumption rate (OCR) and glycolysis by analyzing the extracellular acidification rate (ECAR) via real-time live cell analysis. Using trophoblast cell lines, this protocol focuses on analyzing mitochondrial respiration for both cytotrophoblasts and syncytiotrophoblasts. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 29 November 2023.
All research outputs
#22,306,880
of 24,896,578 outputs
Outputs from Methods in molecular biology
#10,743
of 13,982 outputs
Outputs of similar age
#40,975
of 51,920 outputs
Outputs of similar age from Methods in molecular biology
#77
of 113 outputs
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So far Altmetric has tracked 13,982 research outputs from this source. They receive a mean Attention Score of 3.5. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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